Selection of Internal Reference Genes for SYBR Green QRT-PCR Studies of Rhesus Monkey (Macaca Mulatta) Tissues

Overview

Journal BMC Mol Biol

Publisher Biomed Central

Specialty Molecular Biology

Date 2008 Sep 11

PMID 18782457

Citations 53

Authors

Kung Ahn

Jae-Won Huh

Sang-Je Park

Dae-Soo Kim

Hong-Seok Ha

Yun-Ji Kim

Ja-Rang Lee

Kyu-Tae Chang

Heui-Soo Kim

Affiliations

Soon will be listed here.

Abstract

Background: The rhesus monkey (Macaca mulatta) is a valuable and widely used model animal for biomedical research. However, quantitative analyses of rhesus gene expression profiles under diverse experimental conditions are limited by a shortage of suitable internal controls for the normalization of mRNA levels. In this study, we used a systematic approach for the selection of potential reference genes in the rhesus monkey and compared their suitability to that of the corresponding genes in humans.

Results: Eight housekeeping genes (HKGs) (GAPDH, SDHA, ACTB, RPL13A, RPL32, UBA52, PGK1Y, and YWHAZ) from rhesus monkeys and humans were selected to test for normalization of expression levels in six different tissue types (brain, colon, kidney, liver, lung, and stomach). Their stability and suitability as reference genes were validated by geNorm, NormFinder and BestKeeper programs. Intriguingly, RPL13A and RPL32 were selected as ideal reference genes only in rhesus monkeys.

Conclusion: The results clearly indicated the necessity of using different reference genes for normalization of expression levels between rhesus monkeys and humans in various tissues.

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