Integration of Rous Sarcoma Virus DNA: a CA Dinucleotide is Not Required for Integration of the U3 End of Viral DNA

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2008 Sep 5

PMID 18768972

Citations 4

Authors

Jangsuk Oh

Kevin W Chang

Stephen H Hughes

Affiliations

Soon will be listed here.

Abstract

The two ends of RSV linear DNA are independently inserted into host DNA by integrase in vivo. We previously showed that the range of U3 sequences that are acceptable substrates for integrase appeared to be greater than the range of acceptable U5 sequences in vivo. We have done additional experiments to determine which U3 sequences are good integrase substrates. On the U3 end, there does not appear to be a stringent requirement for the canonical CA, integrase can efficiently remove three nucleotides, and six nucleotides are sufficient to allow integration with reasonable, albeit reduced, efficiency.

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