Quantitative Evaluation of Microvascular Density After Stroke in Rats Using MRI

Overview

Journal J Cereb Blood Flow Metab

Publisher Sage Publications

Specialties Cardiology & Vascular Diseases
Endocrinology
Neurology

Date 2008 Sep 4

PMID 18766197

Citations 16

Authors

Asamoah Bosomtwi

Quan Jiang

Guang L Ding

Li Zhang

Zheng G Zhang

Mei Lu

James R Ewing

Michael Chopp

Affiliations

Soon will be listed here.

Abstract

We investigated vascular changes after stroke using magnetic resonance imaging (MRI) microvascular density (MVD) measurement. T(2) and T(2)(*) were measured in eight rats before and after injecting an intravascular superparamagnetic iron oxide contrast agent to derive the corresponding transverse relaxation shift. Reliability of MRI for measurement of MVD was compared with corresponding sections immunostained with von Willebrand factor (vWF) 2 weeks after stroke. The intracorrelation coefficient (ICC) and its 95% lower bound (LB) was high in the ischemic recovery region (ICC=0.753), moderate in the contralateral area of normal brain tissue (ICC=0.70), and low in the ischemic core (ICC=0.24). A very good agreement (ICC=0.85) and correlation (r=0.90) were observed using only the recovery region and normal contralateral hemisphere (ICC=0.85; 95% LB=0.78; P<0.05). The mean MRI MVD in the center of the core lesion (26+/-9 per mm(2)) was lower than in the recovery region (209+/-60 per mm(2)) or contralateral normal hemisphere (313+/-32 per mm(2)). However, large errors in MRI MVD were encountered in the ischemic core. Our data demonstrate that MRI MVD measurements can quantitatively evaluate microvascular changes in the brain tissue after stroke, if the MVD is not extremely low as in the ischemic core.

Citing Articles

MRI investigation of vascular remodeling for heterogeneous edema lesions in subacute ischemic stroke rat models: Correspondence between cerebral vessel structure and function.

Kang M, Jin S, Cho H J Cereb Blood Flow Metab. 2021; 41(12):3273-3287.

PMID: 34233533 PMC: 8669276. DOI: 10.1177/0271678X211029197.

Magnetic resonance imaging-based changes in vascular morphology and cerebral perfusion in subacute ischemic stroke.

Kufner A, Khalil A, Galinovic I, Kellner E, Mekle R, Rackoll T J Cereb Blood Flow Metab. 2021; 41(10):2617-2627.

PMID: 33866849 PMC: 8504415. DOI: 10.1177/0271678X211010071.

MRI Visualization of Whole Brain Macro- and Microvascular Remodeling in a Rat Model of Ischemic Stroke: A Pilot Study.

Kang M, Jin S, Lee D, Cho H Sci Rep. 2020; 10(1):4989.

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Preclinical Imaging Biomarkers for Postischaemic Neurovascular Remodelling.

Gandhi R, Tsoumpas C Contrast Media Mol Imaging. 2019; 2019:3128529.

PMID: 30863220 PMC: 6378027. DOI: 10.1155/2019/3128529.

Translational MR Neuroimaging of Stroke and Recovery.

Mandeville E, Ayata C, Zheng Y, Mandeville J Transl Stroke Res. 2016; 8(1):22-32.

PMID: 27578048 PMC: 5243530. DOI: 10.1007/s12975-016-0497-z.

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