Methylation-specific Multiplex Ligation-dependent Probe Amplification in Meningiomas

Overview

Journal J Neurooncol

Publisher Springer

Date 2008 Sep 3

PMID 18763019

Citations 6

Authors

Christian Ewald

Thomas Hofmann

Susanne A Kuhn

Thomas Deufel

Christian Beetz

Rolf Kalff

Affiliations

Soon will be listed here.

Abstract

Genomic loss and promotor methylation contribute to inactivation of tumor suppressor genes (TSGs). Methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA) is a relatively new method for simultaneous detection of both these alterations. Here, we apply MS-MLPA to a series of 15 meningiomas of different WHO grades. The two MS-MLPA probe sets used detect copy number changes in 55 unselected TSGs and promotor methylation in a subset of 36 of these genes. Our findings concerning genomic deletions are concordant with previously published studies using alternative techniques. The number of aberrations identified per tumor increased with histopathologically determined grading. The most frequent single event was deletion of the von Hippel-Lindau (VHL) gene in 12 of the 15 tumors. Moreover, VHL deletion status was associated with presence/absence of peritumoral edema. Methylation was rare, being observed in only four tumors and in each case restricted to a single gene. We conclude that a meningioma-specific MS-MLPA probe set would be a valuable tool for both research and diagnostic approaches in these tumors.

Citing Articles

Alterations of oxidative phosphorylation in meningiomas and peripheral nerve sheath tumors.

Feichtinger R, Weis S, Mayr J, Zimmermann F, Bogner B, Sperl W Neuro Oncol. 2015; 18(2):184-94.

PMID: 26106125 PMC: 4724172. DOI: 10.1093/neuonc/nov105.

Methylation-specific multiplex ligation-dependent probe amplification and its impact on clinical findings in medulloblastoma.

Feierabend D, Walter J, Grube S, Herbold C, Beetz C, Kalff R J Neurooncol. 2013; 116(2):213-20.

PMID: 24162828 DOI: 10.1007/s11060-013-1286-0.

Diagnostic utility of MS-MLPA in DNA methylation profiling of adenocarcinomas and neuroendocrine carcinomas of the colon-rectum.

Furlan D, Sahnane N, Mazzoni M, Pastorino R, Carnevali I, Stefanoli M Virchows Arch. 2012; 462(1):47-56.

PMID: 23224118 DOI: 10.1007/s00428-012-1348-2.

Use of the MLPA assay in the molecular diagnosis of gene copy number alterations in human genetic diseases.

Stuppia L, Antonucci I, Palka G, Gatta V Int J Mol Sci. 2012; 13(3):3245-3276.

PMID: 22489151 PMC: 3317712. DOI: 10.3390/ijms13033245.

Multiplexed methylation profiles of tumor suppressor genes in bladder cancer.

Cabello M, Grau L, Franco N, Orenes E, Alvarez M, Blanca A J Mol Diagn. 2011; 13(1):29-40.

PMID: 21227392 PMC: 3070573. DOI: 10.1016/j.jmoldx.2010.11.008.

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