Evaluation of Microarray Surfaces and Arraying Parameters for Autoantibody Profiling

Overview

Journal Proteomics

Date 2008 Aug 30

PMID 18752214

Citations 18

Authors

Imelda Balboni

Cindy Limb

Jessica D Tenenbaum

Paul J Utz

Affiliations

Soon will be listed here.

Abstract

Autoantigen microarrays are being used increasingly to study autoimmunity. Significant variation has been observed when comparing microarray surfaces, printing methods, and probing conditions. In the present study, 24 surfaces and several arraying parameters were analyzed using >500 feature autoantigen microarrays printed with quill pins. A small subset of slides, including FAST, PATH, and SuperEpoxy2, performed well while maintaining the sensitivity and specificity of autoantigen microarrays previously demonstrated by our laboratory. By optimizing the major variables in our autoantigen microarray platform, subtle differences in serum samples can be identified that will shed light on disease pathogenesis.

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