Cryoenzymology in Mixed Solvents Without Cosolvent Effects on Enzyme Specific Activity

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Jun 1

PMID 18734

Citations 3

Authors

P Douzou

C Balny

Affiliations

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Abstract

Water-soluble polyelectrolytes in interaction with proteins are described. These polyelectrolytes make it possible to investigate enzyme-catalyzed reactions in cooled mixed solvents without the usual effects of their organic solvent component on enzyme specific activity. The applicability of techniques developed is illustrated by results obtained on several systems. The possibility of an electrostatic "sorting out" of solvents and its potentialities in cryoenzymology are discussed.

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