Effects of Neuroglobin Overexpression on Mitochondrial Function and Oxidative Stress Following Hypoxia/reoxygenation in Cultured Neurons

Overview

Journal J Neurosci Res

Specialty Neurology

Date 2008 Aug 20

PMID 18711728

Citations 59

Authors

Jianxiang Liu

Zhanyang Yu

Shuzhen Guo

Sun-Ryung Lee

Changhong Xing

Chenggang Zhang

Yan Gao

David G Nicholls

Eng H Lo

Xiaoying Wang

Affiliations

Soon will be listed here.

Abstract

Neuroglobin (Ngb) is a recently discovered tissue globin with a high affinity for oxygen that is widely and specifically expressed in neurons of vertebrate central and peripheral nervous systems. Our laboratory and others have shown Ngb overexpression can protect neurons against hypoxic/ischemic insults, but the underlying mechanisms remain poorly understood. In this study, we examined the effects of Ngb overexpression on mitochondrial function, oxidative stress, and neurotoxicity in primary cortical neurons following hypoxia/reoxygenation (H/R). Ngb-overexpressing transgenic neurons (Ngb-Tg) were significantly protected against H/R-induced cell death. Rates of decline in ATP levels, MTT reduction, and mitochondrial membrane potential were significantly ameliorated in Ngb-Tg neurons. Furthermore, Ngb overexpression reduced superoxide anion generation after H/R, whereas glutathione levels were significantly improved compared with WT controls. Taken together, these data suggest that Ngb is neuroprotective against hypoxia, in part by improving mitochondria function and decreasing oxidative stress.

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