Deficit of Kcnma1 MRNA Expression in the Dentate Gyrus of Epileptic Rats

Overview

Journal Neuroreport

Specialty Neurology

Date 2008 Aug 13

PMID 18695509

Citations 17

Authors

Boris Ermolinsky

Massoud F Arshadmansab

Luis F Pacheco Otalora

Masoud M Zarei

Emilio R Garrido-Sanabria

Affiliations

Soon will be listed here.

Abstract

Epileptogenesis in mesial temporal lobe epilepsy is determined by several factors including abnormalities in the expression and function of ion channels. Here, we report a long-lasting deficit in gene expression of Kcnma1 coding for the large-conductance calcium-activated potassium (BK, MaxiK) channel alpha-subunits after pilocarpine-induced status epilepticus. By using comparative real-time PCR, Taqman gene expression assays, and the delta-delta comparative threshold method we detected a significant reduction in Kcnma1 expression in microdissected dentate gyrus at different intervals after status epilepticus (24 h, 10 days, 1 month, and more than 2 months). BK channels are key regulators of neuronal excitability and transmitter release. Hence, defective Kcnma1 expression may play a critical role in the pathogenesis of mesial temporal lobe epilepsy.

Citing Articles

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