Assessment of Functional Recovery and Axonal Sprouting in Oligodendrocyte-myelin Glycoprotein (OMgp) Null Mice After Spinal Cord Injury

Overview

Journal Mol Cell Neurosci

Specialties Cell Biology
Molecular Biology
Neurology

Date 2008 Aug 12

PMID 18692574

Citations 33

Authors

Benxiu Ji

Lauren C Case

Kai Liu

Zhaohui Shao

Xinhua Lee

Zhongshu Yang

Joy Wang

Tim Tian

Svetlana Shulga-Morskaya

Martin Scott

Zhigang He

Jane K Relton

Sha Mi

Affiliations

Soon will be listed here.

Abstract

Oligodendrocyte-myelin glycoprotein (OMgp) is a myelin component that has been shown in vitro to inhibit neurite outgrowth by binding to the Nogo-66 receptor (NgR1)/Lingo-1/Taj (TROY)/p75 receptor complex to activate the RhoA pathway. To investigate the effects of OMgp on axon regeneration in vivo, OMgp(-/-) mice on a mixed 129/Sv/C57BL/6 (129BL6) or a C57BL/6 (BL6) genetic background were tested in two spinal cord injury (SCI) models - a severe complete transection or a milder dorsal hemisection. OMgp(-/-) mice on the mixed 129BL6 genetic background showed greater functional improvement compared to OMgp(+/+) littermates, with increased numbers of cholera toxin B-labeled ascending sensory axons and 5-HT(+) descending axons and less RhoA activation after spinal cord injury. Myelin isolated from OMgp(-/-) mice (129BL6) was significantly less inhibitory to neurite outgrowth than wild-type (wt) myelin in vitro. However, OMgp(-/-) mice on a BL/6 genetic background showed neither statistically significant functional recovery nor axonal sprouting following dorsal hemisection.

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