Inhibitory Effects of Ovomacroglobulin on Bacterial Keratitis in Rabbits

Overview

Journal Graefes Arch Clin Exp Ophthalmol

Specialty Ophthalmology

Date 1991 Jan 1

PMID 1869068

Citations 4

Authors

S Miyagawa

R Kamata

K Matsumoto

R Okamura

H Maeda

Affiliations

Soon will be listed here.

Abstract

We studied the inhibitory effects of chicken egg-white ovomacroglobulin (ovoM) on keratitis induced by 56,000-Da protease (56 KP) of Serratia marcescens and by elastase (PE) and alkaline protease (PAP) of Pseudomonas aeruginosa. The effects of ovoM on the serratial and pseudomonal keratitis in rabbits were also elucidated. In one model, four drops of 56 KP, PE, or PAP (1 mg/ml) were applied to wounded corneas of eight eyes. Thereafter, 80 microliters ovoM (10 mg/ml) was dropped into four eyes and 0.01 M phosphate-buffed 0.15 M saline (pH 7.4) into the other eyes as a control. The other in vivo test system involved intrastromal injection of S. marcescens or P. aeruginosa, by which each sample (10(5)-10(7) colony-forming units) mixed with ovoM was injected into one cornea and the other cornea received organisms without ovoM. OvoM completely inhibited the activity of these bacterial proteases in vitro and reduced corneal destruction in experimental keratitis in rabbits. In addition, greatly accelerated wound healing was observed.

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The role of Pseudomonas aeruginosa elastase in corneal ring abscess formation in pseudomonal keratitis.

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Therapeutic intervention with chicken egg white ovomacroglobulin and a new quinolone on experimental Pseudomonas keratitis.

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Suppression of polymorphonuclear leucocyte chemotaxis by Pseudomonas aeruginosa elastase in vitro: a study of the mechanisms and the correlation with ring abscess in pseudomonal keratitis.

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References

Maeda H . Assay of proteolytic enzymes by the fluorescence polarization technique. Anal Biochem. 1979; 92(1):222-7. DOI: 10.1016/0003-2697(79)90649-3. View

Kamata R, Yamamoto T, Matsumoto K, Maeda H . A serratial protease causes vascular permeability reaction by activation of the Hageman factor-dependent pathway in guinea pigs. Infect Immun. 1985; 48(3):747-53. PMC: 261250. DOI: 10.1128/iai.48.3.747-753.1985. View

Kreger A, Gray L . Purification of Pseudomonas aeruginosa proteases and microscopic characterization of pseudomonal protease-induced rabbit corneal damage. Infect Immun. 1978; 19(2):630-48. PMC: 414129. DOI: 10.1128/iai.19.2.630-648.1978. View

Osada T, Sasaki T, Ikai A . Purification and characterization of alpha-macroglobulin and ovomacroglobulin of the green turtle (Chelonia mydas japonica). J Biochem. 1988; 103(2):212-7. DOI: 10.1093/oxfordjournals.jbchem.a122250. View

Molla A, Matsumura Y, Yamamoto T, Okamura R, Maeda H . Pathogenic capacity of proteases from Serratia marcescens and Pseudomonas aeruginosa and their suppression by chicken egg white ovomacroglobulin. Infect Immun. 1987; 55(10):2509-17. PMC: 260738. DOI: 10.1128/iai.55.10.2509-2517.1987. View

Laibson P . Cornea and sclera. Arch Ophthalmol. 1972; 88(5):553-74. DOI: 10.1001/archopht.1972.01000030555018. View

ALLANSMITH M, Whitney C, McClellan B, Newman L . Immunoglobulins in the human eye. Location, type, and amount. Arch Ophthalmol. 1973; 89(1):36-45. DOI: 10.1001/archopht.1973.01000040038010. View

Lass J, Haaf J, Foster C, Belcher C . Visual outcome in eight cases of Serratia marcescens keratitis. Am J Ophthalmol. 1981; 92(3):384-90. DOI: 10.1016/0002-9394(81)90529-8. View

Molla A, Tanase S, Hong Y, Maeda H . Interdomain cleavage of plasma fibronectin by zinc-metalloproteinase from Serratia marcescens. Biochim Biophys Acta. 1988; 955(1):77-85. DOI: 10.1016/0167-4838(88)90181-1. View

10.

Nishigai M, Osada T, Ikai A . Structural changes in alpha-2- and ovomacroglobulins studied by gel chromatography and electron microscopy. Biochim Biophys Acta. 1985; 831(2):236-41. DOI: 10.1016/0167-4838(85)90040-8. View

11.

Bjorck L, Akesson P, Bohus M, Trojnar J, Abrahamson M, Olafsson I . Bacterial growth blocked by a synthetic peptide based on the structure of a human proteinase inhibitor. Nature. 1989; 337(6205):385-6. DOI: 10.1038/337385a0. View

12.

Kamata R, Matsumoto K, Okamura R, Yamamoto T, Maeda H . The serratial 56K protease as a major pathogenic factor in serratial keratitis. Clinical and experimental study. Ophthalmology. 1985; 92(10):1452-9. DOI: 10.1016/s0161-6420(85)33855-1. View

13.

Matsumoto K, Maeda H, Takata K, Kamata R, Okamura R . Purification and characterization of four proteases from a clinical isolate of Serratia marcescens kums 3958. J Bacteriol. 1984; 157(1):225-32. PMC: 215156. DOI: 10.1128/jb.157.1.225-232.1984. View

14.

Heck L, Morihara K, McRae W, Miller E . Specific cleavage of human type III and IV collagens by Pseudomonas aeruginosa elastase. Infect Immun. 1986; 51(1):115-8. PMC: 261073. DOI: 10.1128/iai.51.1.115-118.1986. View

15.

Molla A, Yamamoto T, Akaike T, Miyoshi S, Maeda H . Activation of hageman factor and prekallikrein and generation of kinin by various microbial proteinases. J Biol Chem. 1989; 264(18):10589-94. View

16.

Molla A, Matsumoto K, Oyamada I, Katsuki T, Maeda H . Degradation of protease inhibitors, immunoglobulins, and other serum proteins by Serratia protease and its toxicity to fibroblast in culture. Infect Immun. 1986; 53(3):522-9. PMC: 260821. DOI: 10.1128/iai.53.3.522-529.1986. View

17.

Ikai A, Kitamoto T, Nishigai M . Alpha-2-macroglobulin-like protease inhibitor from the egg white of cuban crocodile (Crocodylus rhombifer). J Biochem. 1983; 93(1):121-7. DOI: 10.1093/oxfordjournals.jbchem.a134145. View

18.

Molla A, Kagimoto T, Maeda H . Cleavage of immunoglobulin G (IgG) and IgA around the hinge region by proteases from Serratia marcescens. Infect Immun. 1988; 56(4):916-20. PMC: 259390. DOI: 10.1128/iai.56.4.916-920.1988. View

19.

Steuhl K, DORING G, Henni A, Thiel H, Botzenhart K . Relevance of host-derived and bacterial factors in Pseudomonas aeruginosa corneal infections. Invest Ophthalmol Vis Sci. 1987; 28(9):1559-68. View

20.

Parment P, Ronnerstam R . Soft contact lens keratitis associated with Serratia marcescens. Acta Ophthalmol (Copenh). 1981; 59(4):560-5. DOI: 10.1111/j.1755-3768.1981.tb08343.x. View