Role of MAPK in Mechanical Force-induced Up-regulation of Type I Collagen and Osteopontin in Human Gingival Fibroblasts

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2008 Aug 7

PMID 18682895

Citations 17

Authors

Young-Mi Jeon

Sung-Ho Kook

Young-Ok Son

Eun Mi Kim

Soon-Sun Park

Jong-Ghee Kim

Jeong-Chae Lee

Affiliations

Soon will be listed here.

Abstract

In addition to periodontal ligament, the gingival plays an important role in alveolar bone remodeling induced by physiological and mechanical stimuli. However, there are few reports showing the cellular responses of human gingival fibroblasts (HGF) to a mechanical force. This study examined the effects of centrifugal force on the proliferation of the bone tissue components, such as type I collagen (COL I), osteopontin (OPN), and osteonectin (ONN) in the HGF. The roles of extracellular signal-regulated kinase (ERK), c-Jun-N-terminal kinase (JNK), and p-38 kinase were also investigated. Centrifugal force induced cell cycle arrest in the G(1) phase without any cytotoxic effects and increased the levels of COL I and OPN expression in the cells but had no effect on ONN. The force-induced up-regulation of COL I was found to be mediated by both the ERK-c-Fos-COL I and JNK-c-Jun-COL I pathways, while that of OPN was mediated only by the ERK-mediated pathway. Our present findings suggest that centrifugal force up-regulates COL I and OPN expression in HGF, where both ERK and JNK play indispensable roles.

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