Regulation of Mitochondrial Protein Synthesis by Cytoplasmic Proteins

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1976 Nov 1

PMID 186780

Citations 16

Authors

R O Poyton

J Kavanagh

Affiliations

Soon will be listed here.

Abstract

Isolated yeast mitochondria, which synthesize identifiable polypeptides identical to those made in vivo, have been used in an invitro system to study cytoplasmic control of mitochondrial protein synthesis. It has been found that protein synthesis in isolated mitochondria is dependent on an endogenous pool of cytoplasmically synthesized proteins present within mitochondria at the time of isolation, that protein synthesis ceases apparently when this pool of proteins is depleted, and that a cytoplasmic extract can restore protein synthesis in depleted mitochondria. By use of depleted mitochondria to assay for stimulatory factors it has been found that the bulk of the stimulatory activity in the cytoplasm is of a protein nature and resides predominantly in the postpolysomal supernatant. At least one cytoplasmic stimulatory protein appears to exert a specific effect on the synthesis of subunits I-III of cytochrome c oxidase (ferrocytochrome c:oxygen oxidoreductase; EC 1.9.3.1).

Citing Articles

In Vitro Study of Mitochondrial Protein Synthesis during Mitochondrial Biogenesis in Excised Plant Storage Tissue.

Forde B, Oliver R, Leaver C Plant Physiol. 1979; 63(1):67-73.

PMID: 16660696 PMC: 542768. DOI: 10.1104/pp.63.1.67.

Nuclear and cytoplasmic genes controlling synthesis of variant mitochondrial polypeptides in male-sterile maize.

Forde B, Leaver C Proc Natl Acad Sci U S A. 1980; 77(1):418-22.

PMID: 16592757 PMC: 348282. DOI: 10.1073/pnas.77.1.418.

Evidence for the sequential assembly of cytochrome oxidase subunits in rat liver mitochondria.

Wielburski A, Nelson B Biochem J. 1983; 212(3):829-34.

PMID: 6309154 PMC: 1153160. DOI: 10.1042/bj2120829.

The four cytoplasmically made subunits of yeast mitochondrial cytochrome c oxidase are synthesized individually and not as a polyprotein.

Mihara K, Blobel G Proc Natl Acad Sci U S A. 1980; 77(7):4160-4.

PMID: 6254013 PMC: 349790. DOI: 10.1073/pnas.77.7.4160.

Cytoplasmically made subunits of yeast mitochondrial F1-ATPase and cytochrome c oxidase are synthesized as individual precursors, not as polyproteins.

Lewin A, Gregor I, Mason T, Nelson N, Schatz G Proc Natl Acad Sci U S A. 1980; 77(7):3998-4002.

PMID: 6254007 PMC: 349755. DOI: 10.1073/pnas.77.7.3998.

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