Spatial and Circadian Regulation of Cry in Drosophila

Overview

Journal J Biol Rhythms

Date 2008 Jul 30

PMID 18663236

Citations 7

Authors

Hao Zheng

Fanny Ng

Yixiao Liu

Paul E Hardin

Affiliations

Soon will be listed here.

Abstract

In Drosophila, cryptochrome (cry) encodes a blue-light photoreceptor that mediates light input to circadian oscillators and sustains oscillator function in peripheral tissues. The levels of cry mRNA cycle with a peak at approximately ZT5, which is similar to the phase of Clock (Clk) mRNA cycling in Drosophila. To understand how cry spatial and circadian expression is regulated, a series of cry-Gal4 trans-genes containing different portions of cry upstream and intron 1 sequences were tested for spatial and circadian expression. In fly heads, cry upstream sequences drive constitutive expression in brain oscillator neurons, a novel group of nonoscillator cells in the optic lobe, and peripheral oscillator cells in eyes and antennae. In contrast, cry intron 1 drives rhythmic expression in eyes and antennae, but not brain oscillator neurons. These results demonstrate that intron 1 is sufficient for high-amplitude cry mRNA cycling, show that cry upstream sequences are sufficient for expression in brain oscillator neurons, and suggest that cry spatial and circadian expression are regulated by different elements.

Citing Articles

Molecular Characterization and Expression Profiles of Cryptochrome Genes in a Long-Distance Migrant, Agrotis segetum (Lepidoptera: Noctuidae).

Chang H, Guo J, Fu X, Wang M, Hou Y, Wu K J Insect Sci. 2019; 19(1).

PMID: 30690535 PMC: 6342827. DOI: 10.1093/jisesa/iey127.

CLOCK stabilizes CYCLE to initiate clock function in .

Liu T, Mahesh G, Yu W, Hardin P Proc Natl Acad Sci U S A. 2017; 114(41):10972-10977.

PMID: 28973907 PMC: 5642697. DOI: 10.1073/pnas.1707143114.

Circadian rhythms in insect disease vectors.

Meireles-Filho A, Kyriacou C Mem Inst Oswaldo Cruz. 2014; 108 Suppl 1:48-58.

PMID: 24473802 PMC: 4109179. DOI: 10.1590/0074-0276130438.

The clock input to the first optic neuropil of Drosophila melanogaster expressing neuronal circadian plasticity.

Damulewicz M, Pyza E PLoS One. 2011; 6(6):e21258.

PMID: 21760878 PMC: 3124489. DOI: 10.1371/journal.pone.0021258.

DN1(p) circadian neurons coordinate acute light and PDF inputs to produce robust daily behavior in Drosophila.

Zhang L, Chung B, Lear B, Kilman V, Liu Y, Mahesh G Curr Biol. 2010; 20(7):591-9.

PMID: 20362452 PMC: 2864127. DOI: 10.1016/j.cub.2010.02.056.

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