Comparison of Intestinal Microflora in Healthy Infants and Infants with Allergic Colitis

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 2008 Jul 29

PMID 18661304

Citations 7

Authors

M Smehilova

E Vlkova

J Nevoral

K Flajsmanova

J Killer

V Rada

Affiliations

Soon will be listed here.

Abstract

Twenty-eight exclusively breast-fed healthy infants and 16 infants also exclusively breast-fed with allergic colitis (aged 85 +/- 60 and 98 +/- 58 d, respectively) were screened for differences in fecal flora. Bifidobacteria were detected in 23 healthy infants and only in 4 fecal samples of infants with allergic colitis. All bifidobacteria-free infants possessed Gram-positive regular rods as a major group of their fecal flora. These bacteria were identified as clostridia using genus-specific FISH probe. Infants with allergy colitis possessed significantly lower counts of bifidobacteria and total anaerobes and significantly higher counts of clostridia in their feces. In healthy infants, Bifidobacterium longum was the most frequently found species (54.5% of the samples), followed by B. adolescentis (20.0), B. breve (18.2), B. bifidum (16.4), B. dentium (10.9) and B. pseudocatenulatum (1.80). Bifidobacterial isolates from two babies with allergic colitis were identified as B. longum, one child from patients group contained species B. dentium and one baby B. adolescentis. Our results suggest that there are significantly lower counts of bifidobacteria in infants with allergic colitis than in healthy infants.

Citing Articles

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References

Kirjavainen P, Apostolou E, Arvola T, Salminen S, Gibson G, Isolauri E . Characterizing the composition of intestinal microflora as a prospective treatment target in infant allergic disease. FEMS Immunol Med Microbiol. 2001; 32(1):1-7. DOI: 10.1111/j.1574-695X.2001.tb00526.x. View

Millar M, Wilks M, Costeloe K . Probiotics for preterm infants?. Arch Dis Child Fetal Neonatal Ed. 2003; 88(5):F354-8. PMC: 1721619. DOI: 10.1136/fn.88.5.f354. View

McCartney A . Application of molecular biological methods for studying probiotics and the gut flora. Br J Nutr. 2002; 88 Suppl 1:S29-37. DOI: 10.1079/BJN2002627. View

Edwards C, Parrett A . Intestinal flora during the first months of life: new perspectives. Br J Nutr. 2002; 88 Suppl 1:S11-8. DOI: 10.1079/BJN2002625. View

Matsuki T, Watanabe K, Tanaka R, Fukuda M, Oyaizu H . Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted species-specific primers. Appl Environ Microbiol. 1999; 65(10):4506-12. PMC: 91600. DOI: 10.1128/AEM.65.10.4506-4512.1999. View

Howie P, Forsyth J, Ogston S, Clark A, Florey C . Protective effect of breast feeding against infection. BMJ. 1990; 300(6716):11-6. PMC: 1661904. DOI: 10.1136/bmj.300.6716.11. View

Lundequist B, Nord C, Winberg J . The composition of the faecal microflora in breastfed and bottle fed infants from birth to eight weeks. Acta Paediatr Scand. 1985; 74(1):45-51. DOI: 10.1111/j.1651-2227.1985.tb10919.x. View

Mullie C, Romond M, Izard D . Establishment and follow-up of bifidobacterial species in the gut of healthy bottle-fed infants of 1-4 months age. Folia Microbiol (Praha). 2006; 51(5):473-7. DOI: 10.1007/BF02931594. View

Locascio M, Holgado A, Perdigon G, Oliver G . Enteric bifidobacteria: isolation from human infants and challenge studies in mice. Can J Microbiol. 2002; 47(11):1048-52. DOI: 10.1139/w01-112. View

10.

Orban J, Patterson J . Modification of the phosphoketolase assay for rapid identification of bifidobacteria. J Microbiol Methods. 2000; 40(3):221-4. DOI: 10.1016/s0167-7012(00)00133-0. View

11.

Ouwehand A, Isolauri E, He F, Hashimoto H, Benno Y, Salminen S . Differences in Bifidobacterium flora composition in allergic and healthy infants. J Allergy Clin Immunol. 2001; 108(1):144-5. DOI: 10.1067/mai.2001.115754. View

12.

Vlkova E, Trojanova I, Rada V . Distribution of bifidobacteria in the gastrointestinal tract of calves. Folia Microbiol (Praha). 2006; 51(4):325-8. DOI: 10.1007/BF02931825. View

13.

Kocourkova I, Zadnikova R, Zizka J, Rosova V . Effect of oral application of a probiotic E. coli strain on the intestinal microflora of children of allergic mothers during the first year of life. Folia Microbiol (Praha). 2007; 52(2):189-93. DOI: 10.1007/BF02932158. View

14.

Rada V, Petr J . A new selective medium for the isolation of glucose non-fermenting bifidobacteria from hen caeca. J Microbiol Methods. 2000; 43(2):127-32. DOI: 10.1016/s0167-7012(00)00205-0. View

15.

Fanaro S, Chierici R, Guerrini P, Vigi V . Intestinal microflora in early infancy: composition and development. Acta Paediatr Suppl. 2003; 91(441):48-55. DOI: 10.1111/j.1651-2227.2003.tb00646.x. View

16.

Trebichavsky I, Splichal I . Probiotics manipulate host cytokine response and induce antimicrobial peptides. Folia Microbiol (Praha). 2006; 51(5):507-10. DOI: 10.1007/BF02931599. View