Multilayer Nanofilms As Substrates for Hepatocellular Applications

Overview

Journal Biomaterials

Specialty Biomedical Engineering

Date 2008 Jul 26

PMID 18653230

Citations 15

Authors

Corinne R Wittmer

Jennifer A Phelps

Christin M Lepus

William M Saltzman

Martha J Harding

Paul R Van Tassel

Affiliations

Soon will be listed here.

Abstract

Multilayer nanofilms, formed by the layer-by-layer (LbL) adsorption of positively and negatively charged polyelectrolytes, are promising substrates for tissue engineering. We investigate here the attachment and function of hepatic cells on multilayer films in terms of film composition, terminal layer, rigidity, charge, and presence of biofunctional species. Human hepatocellular carcinoma (HepG2) cells, adult rat hepatocytes (ARH), and human fetal hepatoblasts (HFHb) are studied on films composed of the polysaccharides chitosan (CHI) and alginate (ALG), the polypeptides poly(l-lysine) (PLL) and poly(l-glutamic acid) (PGA), and the synthetic polymers poly(allylamine hydrochloride) (PAH) and poly(styrene sulfonate) (PSS). The influence of chemical cross-linking following LbL assembly is also investigated. We find HepG2 to reach confluence after 7 days of culture on only 2 of 18 candidate multilayer systems: (PAH-PSS)(n) (i.e. nPAH-PSS bilayers) and cross-linked (PLL-ALG)(n)-PLL. Cross-linked PLL-ALG and PLL-PGA films support attachment and function of ARH, independently of the terminal layer, provided collagen is adsorbed to the top of the film. (PAH-PSS)(n), cross-linked (PLL-ALG)(n), and cross-linked (PLL-PGA)(n)-PLL films all support attachment, layer confluence, and function of HFHb, with the latter film promoting the greatest level of function at 8 days. Overall, film composition, terminal layer, and rigidity are key variables in promoting attachment and function of hepatic cells, while film charge and biofunctionality are somewhat less important. These studies reveal optimal candidate multilayer biomaterials for human liver tissue engineering applications.

Citing Articles

The Modulation of Septic Shock: A Proteomic Approach.

Alves P, de Souza A, Bastos V, Miguel E, Ramos A, Cameron L Int J Mol Sci. 2024; 25(19).

PMID: 39408970 PMC: 11476436. DOI: 10.3390/ijms251910641.

Development of an Improved Method for the Isolation and Culture of Newborn Sheep Primary Hepatocytes.

Chen B, Dou X, Zhang D, Liu T, Yang B, Lu Z Curr Issues Mol Biol. 2022; 44(8):3621-3631.

PMID: 36005144 PMC: 9406642. DOI: 10.3390/cimb44080248.

Hepatic Polarization Accelerated by Mechanical Compaction Involves HNF4 Activation.

Yang J, Liang J, Zheng Y, Li S, Li Y, Liu H Biomed Res Int. 2020; 2020:8016306.

PMID: 32802875 PMC: 7426769. DOI: 10.1155/2020/8016306.

Fibronectin in Layer-by-Layer Assembled Films Switches Tumor Cells between 2D and 3D Morphology.

Bhadriraju K, Hong J, Lund S, Reyes D ACS Biomater Sci Eng. 2019; 3.

PMID: 31093521 PMC: 6513000. DOI: 10.1021/acsbiomaterials.7b00608.

Hierarchy of Hybrid Materials-The Place of Inorganics--Organics in it, Their Composition and Applications.

Saveleva M, Eftekhari K, Abalymov A, Douglas T, Volodkin D, Parakhonskiy B Front Chem. 2019; 7:179.

PMID: 31019908 PMC: 6459030. DOI: 10.3389/fchem.2019.00179.

References

Berg M, Yang S, Hammond P, Rubner M . Controlling mammalian cell interactions on patterned polyelectrolyte multilayer surfaces. Langmuir. 2005; 20(4):1362-8. DOI: 10.1021/la0355489. View

Tryoen-Toth P, Vautier D, Haikel Y, Voegel J, Schaaf P, Chluba J . Viability, adhesion, and bone phenotype of osteoblast-like cells on polyelectrolyte multilayer films. J Biomed Mater Res. 2002; 60(4):657-67. DOI: 10.1002/jbm.10110. View

Boura C, Menu P, Payan E, Picart C, Voegel J, Muller S . Endothelial cells grown on thin polyelectrolyte mutlilayered films: an evaluation of a new versatile surface modification. Biomaterials. 2003; 24(20):3521-30. DOI: 10.1016/s0142-9612(03)00214-x. View

Voros J, Ramsden J, Csucs G, Szendro I, De Paul S, Textor M . Optical grating coupler biosensors. Biomaterials. 2002; 23(17):3699-710. DOI: 10.1016/s0142-9612(02)00103-5. View

Allen J, Bhatia S . Engineering liver therapies for the future. Tissue Eng. 2002; 8(5):725-37. DOI: 10.1089/10763270260424097. View

Semler E, Lancin P, Dasgupta A, Moghe P . Engineering hepatocellular morphogenesis and function via ligand-presenting hydrogels with graded mechanical compliance. Biotechnol Bioeng. 2005; 89(3):296-307. DOI: 10.1002/bit.20328. View

Lukosz W, Clerc D, Nellen P, Stamm C, Weiss P . Output grating couplers on planar optical waveguides as direct immunosensors. Biosens Bioelectron. 1991; 6(3):227-32. DOI: 10.1016/0956-5663(91)80007-k. View

Cima L, Ingber D, Vacanti J, Langer R . Hepatocyte culture on biodegradable polymeric substrates. Biotechnol Bioeng. 1991; 38(2):145-58. DOI: 10.1002/bit.260380207. View

Deurholt T, Bloemendaal L, Chhatta A, van Wijk A, Weijer K, Seppen J . In vitro functionality of human fetal liver cells and clonal derivatives under proliferative conditions. Cell Transplant. 2007; 15(8-9):811-22. DOI: 10.3727/000000006783464417. View

10.

Chan C, Berthiaume F, Nath B, Tilles A, Toner M, Yarmush M . Hepatic tissue engineering for adjunct and temporary liver support: critical technologies. Liver Transpl. 2004; 10(11):1331-42. DOI: 10.1002/lt.20229. View

11.

Kreke M, Badami A, Brady J, Akers R, Goldstein A . Modulation of protein adsorption and cell adhesion by poly(allylamine hydrochloride) heparin films. Biomaterials. 2004; 26(16):2975-81. DOI: 10.1016/j.biomaterials.2004.08.013. View

12.

Davis M, Vacanti J . Toward development of an implantable tissue engineered liver. Biomaterials. 1996; 17(3):365-72. DOI: 10.1016/0142-9612(96)85575-x. View

13.

Kidambi S, Lee I, Chan C . Controlling primary hepatocyte adhesion and spreading on protein-free polyelectrolyte multilayer films. J Am Chem Soc. 2004; 126(50):16286-7. DOI: 10.1021/ja046188u. View

14.

Kidambi S, Sheng L, Yarmush M, Toner M, Lee I, Chan C . Patterned co-culture of primary hepatocytes and fibroblasts using polyelectrolyte multilayer templates. Macromol Biosci. 2007; 7(3):344-53. DOI: 10.1002/mabi.200600205. View

15.

Dan Y, Riehle K, Lazaro C, Teoh N, Haque J, Campbell J . Isolation of multipotent progenitor cells from human fetal liver capable of differentiating into liver and mesenchymal lineages. Proc Natl Acad Sci U S A. 2006; 103(26):9912-7. PMC: 1502553. DOI: 10.1073/pnas.0603824103. View

16.

Mooney D, Hansen L, Vacanti J, Langer R, Farmer S, Ingber D . Switching from differentiation to growth in hepatocytes: control by extracellular matrix. J Cell Physiol. 1992; 151(3):497-505. DOI: 10.1002/jcp.1041510308. View

17.

Lazaro C, Croager E, Mitchell C, Campbell J, Yu C, Foraker J . Establishment, characterization, and long-term maintenance of cultures of human fetal hepatocytes. Hepatology. 2003; 38(5):1095-106. DOI: 10.1053/jhep.2003.50448. View

18.

Tan W, Desai T . Microscale multilayer cocultures for biomimetic blood vessels. J Biomed Mater Res A. 2004; 72(2):146-60. DOI: 10.1002/jbm.a.30182. View

19.

Park I, Yang J, Jeong H, Bom H, Harada I, Akaike T . Galactosylated chitosan as a synthetic extracellular matrix for hepatocytes attachment. Biomaterials. 2003; 24(13):2331-7. DOI: 10.1016/s0142-9612(03)00108-x. View

20.

Wilson C, Clegg R, Leavesley D, Pearcy M . Mediation of biomaterial-cell interactions by adsorbed proteins: a review. Tissue Eng. 2005; 11(1-2):1-18. DOI: 10.1089/ten.2005.11.1. View