Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes

Overview

Journal Curr Genomics

Publisher Bentham Science Publishers

Date 2008 Jul 23

PMID 18645596

Citations 59

Authors

Sa Deepak

Kr Kottapalli

R Rakwal

G Oros

Ks Rangappa

H Iwahashi

Y Masuo

Gk Agrawal

Affiliations

Soon will be listed here.

Abstract

Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21(st) century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.

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