Vapor Inhalation of Alcohol in Rats

Overview

Journal Curr Protoc Neurosci

Date 2008 Jul 18

PMID 18634001

Citations 109

Authors

Nicholas W Gilpin

Heather N Richardson

Maury Cole

George F Koob

Affiliations

Soon will be listed here.

Abstract

Alcohol dependence constitutes a neuroadaptive state critical for understanding alcoholism, and various methods have been utilized to induce alcohol dependence in animals, one of which is alcohol vapor exposure. Alcohol vapor inhalation provides certain advantages over other chronic alcohol exposure procedures that share the ultimate goal of producing alcohol dependence in rats. Chronic alcohol vapor inhalation allows the experimenter to control the dose, duration, and pattern of alcohol exposure. Also, this procedure facilitates testing of somatic and motivational aspects of alcohol dependence. Chronic exposure to alcohol vapor produces increases in alcohol-drinking behavior, increases in anxiety-like behavior, and reward deficits in rats. Alcohol vapor inhalation as a laboratory protocol is flexible, and the parameters of this procedure can be adjusted to accommodate the specific aims of different experiments. This unit describes the options available to investigators using this procedure for dependence induction, when different options are more or less appropriate, and the implications of each.

Citing Articles

Analgesic effect of oxytocin in alcohol-dependent male and female rats.

Marendes J, Muench M, Young C, Ghaly A, Tunstall B Alcohol. 2024; 123:27-38.

PMID: 39716604 PMC: 11875206. DOI: 10.1016/j.alcohol.2024.12.002.

Noradrenergic Mechanisms and Circuitry of Hyperkatifeia in Alcohol Use Disorder.

Varodayan F, Erikson C, Scroger M, Roberto M Biol Psychiatry. 2024; 97(6):580-589.

PMID: 39304172 PMC: 11839382. DOI: 10.1016/j.biopsych.2024.09.009.

Chronic ethanol exposure produces long-lasting, subregion-specific physiological adaptations in RMTg-projecting mPFC neurons.

Przybysz K, Shillinglaw J, Wheeler S, Glover E Neuropharmacology. 2024; 259():110098.

PMID: 39117106 PMC: 11714651. DOI: 10.1016/j.neuropharm.2024.110098.

Enhancing translation: A need to leverage complex preclinical models of addictive drugs to accelerate substance use treatment options.

Corley C, Craig A, Sadek S, Marusich J, Chehimi S, White A Pharmacol Biochem Behav. 2024; 243():173836.

PMID: 39067531 PMC: 11344688. DOI: 10.1016/j.pbb.2024.173836.

Mouse parasubthalamic neurons drive alcohol drinking escalation and behavioral disinhibition.

Kreifeldt M, Okhuarobo A, Dunning J, Lopez C, Macedo G, Sidhu H bioRxiv. 2024; .

PMID: 39026704 PMC: 11257461. DOI: 10.1101/2024.07.06.602357.

References

FALK J, SAMSON H, Winger G . Behavioral maintenance of high concentrations of blood ethanol and physical dependence in the rat. Science. 1972; 177(4051):811-3. DOI: 10.1126/science.177.4051.811. View

Koob G . Animal models of craving for ethanol. Addiction. 2000; 95 Suppl 2:S73-81. DOI: 10.1080/09652140050111663. View

Kampov-Polevoy A, Matthews D, Gause L, Morrow A, Overstreet D . P rats develop physical dependence on alcohol via voluntary drinking: changes in seizure thresholds, anxiety, and patterns of alcohol drinking. Alcohol Clin Exp Res. 2000; 24(3):278-84. View

Cagetti E, Baicy K, Olsen R . Topiramate attenuates withdrawal signs after chronic intermittent ethanol in rats. Neuroreport. 2004; 15(1):207-10. DOI: 10.1097/00001756-200401190-00040. View

Rimondini R, Arlinde C, Sommer W, Heilig M . Long-lasting increase in voluntary ethanol consumption and transcriptional regulation in the rat brain after intermittent exposure to alcohol. FASEB J. 2002; 16(1):27-35. DOI: 10.1096/fj.01-0593com. View

ODell L, Roberts A, Smith R, Koob G . Enhanced alcohol self-administration after intermittent versus continuous alcohol vapor exposure. Alcohol Clin Exp Res. 2004; 28(11):1676-82. DOI: 10.1097/01.alc.0000145781.11923.4e. View

Gatch M, Wallis C, Lal H . Effects of NMDA antagonists on ethanol-withdrawal induced "anxiety" in the elevated plus maze. Alcohol. 1999; 19(3):207-11. DOI: 10.1016/s0741-8329(99)00045-2. View

Slawecki C . Two-choice reaction time performance in Sprague-Dawley rats exposed to alcohol during adolescence or adulthood. Behav Pharmacol. 2006; 17(7):605-14. DOI: 10.1097/01.fbp.0000236272.10418.62. View

Roberts A, Heyser C, Cole M, Griffin P, Koob G . Excessive ethanol drinking following a history of dependence: animal model of allostasis. Neuropsychopharmacology. 2000; 22(6):581-94. DOI: 10.1016/S0893-133X(99)00167-0. View

10.

Gallate J, Morley K, Ambermoon P, McGregor I . The consequences of beer consumption in rats: acute anxiolytic and ataxic effects and withdrawal-induced anxiety. Psychopharmacology (Berl). 2002; 166(1):51-60. DOI: 10.1007/s00213-002-1291-z. View

11.

Funk C, Zorrilla E, Lee M, Rice K, Koob G . Corticotropin-releasing factor 1 antagonists selectively reduce ethanol self-administration in ethanol-dependent rats. Biol Psychiatry. 2006; 61(1):78-86. PMC: 2741496. DOI: 10.1016/j.biopsych.2006.03.063. View

12.

Lumeng L, Li T . The development of metabolic tolerance in the alcohol-preferring P rats: comparison of forced and free-choice drinking of ethanol. Pharmacol Biochem Behav. 1986; 25(5):1013-20. DOI: 10.1016/0091-3057(86)90079-1. View

13.

Gatto G, Murphy J, Waller M, McBride W, Lumeng L, Li T . Chronic ethanol tolerance through free-choice drinking in the P line of alcohol-preferring rats. Pharmacol Biochem Behav. 1987; 28(1):111-5. DOI: 10.1016/0091-3057(87)90021-9. View

14.

MAJCHROWICZ E . Induction of physical dependence upon ethanol and the associated behavioral changes in rats. Psychopharmacologia. 1975; 43(3):245-54. DOI: 10.1007/BF00429258. View

15.

Kliethermes C . Anxiety-like behaviors following chronic ethanol exposure. Neurosci Biobehav Rev. 2005; 28(8):837-50. DOI: 10.1016/j.neubiorev.2004.11.001. View

16.

Hiltunen A, Koechling U, Borg S . Effects of long-term abstinence on psychological functioning: a prospective longitudinal analysis comparing alcohol-dependent patients and healthy volunteers. Alcohol. 1996; 13(5):415-21. DOI: 10.1016/0741-8329(96)81678-8. View

17.

Schulteis G, Markou A, Cole M, Koob G . Decreased brain reward produced by ethanol withdrawal. Proc Natl Acad Sci U S A. 1995; 92(13):5880-4. PMC: 41605. DOI: 10.1073/pnas.92.13.5880. View

18.

Rassnick S, Heinrichs S, Britton K, Koob G . Microinjection of a corticotropin-releasing factor antagonist into the central nucleus of the amygdala reverses anxiogenic-like effects of ethanol withdrawal. Brain Res. 1993; 605(1):25-32. DOI: 10.1016/0006-8993(93)91352-s. View

19.

Roberts A, Heyser C, Koob G . Operant self-administration of sweetened versus unsweetened ethanol: effects on blood alcohol levels. Alcohol Clin Exp Res. 1999; 23(7):1151-7. View

20.

Valdez G, Roberts A, Chan K, Davis H, Brennan M, Zorrilla E . Increased ethanol self-administration and anxiety-like behavior during acute ethanol withdrawal and protracted abstinence: regulation by corticotropin-releasing factor. Alcohol Clin Exp Res. 2002; 26(10):1494-501. DOI: 10.1097/01.ALC.0000033120.51856.F0. View