UV-light Exposed Prion Protein Fails to Form Amyloid Fibrils

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2008 Jul 17

PMID 18628989

Citations 3

Authors

Abhay Kumar Thakur

Ch Mohan Rao

Affiliations

Soon will be listed here.

Abstract

Amyloid fibril formation involves three steps; structural perturbation, nucleation and elongation. We have investigated amyloidogenesis using prion protein as a model system and UV-light as a structural perturbant. We find that UV-exposed prion protein fails to form amyloid fibrils. Interestingly, if provided with pre-formed fibrils as seeds, UV-exposed prion protein formed amyloid fibrils albeit with slightly different morphology. Atomic force microscopy and electron microscopic studies clearly show the formation of fibrils under these conditions. Circular dichroism study shows loss in helicity in UV-exposed protein. UV-exposed prion protein fails to form amyloid fibrils. However, it remains competent for fibril extension, suggesting that UV-exposure results in loss of nucleating capability. This work opens up possibility of segregating nucleation and elongation step of amyloidogenesis, facilitating screening of new drug candidates for specifically inhibiting either of these processes. In addition, the work also highlights the importance of light-induced structural and functional alterations which are important in protein based therapeutics.

Citing Articles

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Examining the influence of ultraviolet C irradiation on recombinant human γD-crystallin.

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