Unsaturated Long-chain Fatty Acids Induce the Respiratory Burst of Human Neutrophils and Monocytes in Whole Blood

Overview

Journal Nutr Metab (Lond)

Publisher Biomed Central

Date 2008 Jul 16

PMID 18625045

Citations 7

Authors

Bjorn Juttner

Janina Kroplin

Sina M Coldewey

Lars Witt

Wilhelm A Osthaus

Christian Weilbach

Dirk Scheinichen

Affiliations

Soon will be listed here.

Abstract

Background: It is increasingly recognized that infectious complications in patients treated with total parenteral nutrition (TPN) may be caused by altered immune responses. Neutrophils and monocytes are the first line of defence against bacterial and fungal infection through superoxide anion production during the respiratory burst. To characterize the impact of three different types of lipid solutions that are applied as part of TPN formulations, we investigated the unstimulated respiratory burst activation of neutrophils and monocytes in whole blood.

Methods: Whole blood samples were incubated with LCT (Intralipid(R)), LCT/MCT (Lipofundin(R)) and LCT-MUFA (ClinOleic(R)) in three concentrations (0.06, 0.3 and 0.6 mg ml-1) for time periods up to one hour. Hydrogen peroxide production during the respiratory burst of neutrophils and monocytes was measured by flow cytometry.

Results: LCT and LCT-MUFA induced a hydrogen peroxide production in neutrophils and monocytes without presence of a physiological stimulus in contrast to LCT/MCT.

Conclusion: We concluded that parenteral nutrition containing unsaturated oleic (C18:1) and linoleic (C18:2) acid can induce respiratory burst of neutrophils and monocytes, resulting in an elevated risk of tissue damage by the uncontrolled production of reactive oxygen species. Contradictory observations reported in previous studies may in part be the result of different methods used to determine hydrogen peroxide production.

Citing Articles

The Omega-6 Lipid pathway shift is associated with neutrophil influx and structural lung damage in early cystic fibrosis lung disease.

Slimmen L, Broos J, Manai B, Estevao S, Giera M, Kooij G Clin Transl Immunology. 2024; 13(9):e70000.

PMID: 39286529 PMC: 11403467. DOI: 10.1002/cti2.70000.

Janus kinase inhibitors modify the fatty acid profile of extracellular vesicles and modulate the immune response.

Daza Zapata A, Alvarez K, Vasquez Duque G, Palacio J, Rojas Lopez M Heliyon. 2024; 10(3):e24710.

PMID: 38314280 PMC: 10837569. DOI: 10.1016/j.heliyon.2024.e24710.

A Bittersweet Response to Infection in Diabetes; Targeting Neutrophils to Modify Inflammation and Improve Host Immunity.

Dowey R, Iqbal A, Heller S, Sabroe I, Prince L Front Immunol. 2021; 12:678771.

PMID: 34149714 PMC: 8209466. DOI: 10.3389/fimmu.2021.678771.

Biological and Clinical Aspects of an Olive Oil-Based Lipid Emulsion-A Review.

Cai W, Calder P, Cury-Boaventura M, De Waele E, Jakubowski J, Zaloga G Nutrients. 2018; 10(6).

PMID: 29914122 PMC: 6024782. DOI: 10.3390/nu10060776.

The olive oil-based lipid clinoleic blocks leukocyte recruitment and improves survival during systemic inflammation: a comparative in vivo study of different parenteral lipid emulsions.

Buschmann K, Poeschl J, Braach N, Hudalla H, Kuss N, Frommhold D Mediators Inflamm. 2015; 2015:757059.

PMID: 25767334 PMC: 4341856. DOI: 10.1155/2015/757059.

References

Wu G, Jarstrand C, Nordenstrom J . Phagocyte-induced lipid peroxidation of different intravenous fat emulsions and counteractive effect of vitamin E. Nutrition. 1999; 15(5):359-64. DOI: 10.1016/s0899-9007(99)00052-0. View

Lun A, Schmitt M, Renz H . Phagocytosis and oxidative burst: reference values for flow cytometric assays independent of age. Clin Chem. 2000; 46(11):1836-9. View

Versleijen M, Roelofs H, Preijers F, Roos D, Wanten G . Parenteral lipids modulate leukocyte phenotypes in whole blood, depending on their fatty acid composition. Clin Nutr. 2005; 24(5):822-9. DOI: 10.1016/j.clnu.2005.05.003. View

Wanten G, Rops A, van Emst-de Vries S, Naber T, Willems P . Prompt inhibition of fMLP-induced Ca2+ mobilization by parenteral lipid emulsions in human neutrophils. J Lipid Res. 2002; 43(4):550-6. View

Bellinati-Pires R, Waitzberg D, Salgado M, Carneiro-Sampaio M . Functional alterations of human neutrophils by medium-chain triglyceride emulsions: evaluation of phagocytosis, bacterial killing, and oxidative activity. J Leukoc Biol. 1993; 53(4):404-10. DOI: 10.1002/jlb.53.4.404. View

Wanten G, Geijtenbeek T, Raymakers R, van Kooyk Y, Roos D, Jansen J . Medium-chain, triglyceride-containing lipid emulsions increase human neutrophil beta2 integrin expression, adhesion, and degranulation. JPEN J Parenter Enteral Nutr. 2000; 24(4):228-33. DOI: 10.1177/0148607100024004228. View

Kuijpers T, Tool A, van der Schoot C, Ginsel L, Onderwater J, Roos D . Membrane surface antigen expression on neutrophils: a reappraisal of the use of surface markers for neutrophil activation. Blood. 1991; 78(4):1105-11. View

Emmendorffer A, Hecht M, Lohmann-Matthes M, Roesler J . A fast and easy method to determine the production of reactive oxygen intermediates by human and murine phagocytes using dihydrorhodamine 123. J Immunol Methods. 1990; 131(2):269-75. DOI: 10.1016/0022-1759(90)90198-5. View

Waitzberg D, Bellinati-Pires R, Yamaguchi N, Salgado M, Hypolito I, Soares S . Influence of medium-chain triglyceride-based lipid emulsion on rat polymorphonuclear cell functions. Nutrition. 1996; 12(2):93-9. DOI: 10.1016/0899-9007(96)90706-6. View

10.

Wanten G, Naber A, Kruimel J, Tool A, Roos D, Jansen J . Influence of structurally different lipid emulsions on human neutrophil oxygen radical production. Eur J Clin Invest. 1999; 29(4):357-63. DOI: 10.1046/j.1365-2362.1999.00486.x. View

11.

Smith J, Weidemann M . Further characterization of the neutrophil oxidative burst by flow cytometry. J Immunol Methods. 1993; 162(2):261-8. DOI: 10.1016/0022-1759(93)90391-j. View

12.

Tanaka T, Makino R, Iizuka T, Ishimura Y, Kanegasaki S . Activation by saturated and monounsaturated fatty acids of the O2- -generating system in a cell-free preparation from neutrophils. J Biol Chem. 1988; 263(27):13670-6. View

13.

Schepens M, Roelofs H, Peters W, Wanten G . No evidence for oxidative stress in patients on home parenteral nutrition. Clin Nutr. 2006; 25(6):939-48. DOI: 10.1016/j.clnu.2006.05.001. View

14.

Vowells S, Sekhsaria S, Malech H, Shalit M, Fleisher T . Flow cytometric analysis of the granulocyte respiratory burst: a comparison study of fluorescent probes. J Immunol Methods. 1995; 178(1):89-97. DOI: 10.1016/0022-1759(94)00247-t. View

15.

Wanten G, Naber A, Netea M . Modulation of inflammatory cytokine production by parenteral lipid emulsions. Clin Nutr. 2005; 24(1):164-5. DOI: 10.1016/j.clnu.2004.09.017. View

16.

Hatanaka E, Levada-Pires A, Pithon-Curi T, Curi R . Systematic study on ROS production induced by oleic, linoleic, and gamma-linolenic acids in human and rat neutrophils. Free Radic Biol Med. 2006; 41(7):1124-32. DOI: 10.1016/j.freeradbiomed.2006.06.014. View

17.

Howard L, Ashley C . Management of complications in patients receiving home parenteral nutrition. Gastroenterology. 2003; 124(6):1651-61. DOI: 10.1016/s0016-5085(03)00326-3. View

18.

Wanten G, Janssen F, Naber A . Saturated triglycerides and fatty acids activate neutrophils depending on carbon chain-length. Eur J Clin Invest. 2002; 32(4):285-9. DOI: 10.1046/j.1365-2362.2002.00959.x. View

19.

Schneider S, Fung V, Palmblad J, Babior B . Activity of the leukocyte NADPH oxidase in whole neutrophils and cell-free neutrophil preparations stimulated with long-chain polyunsaturated fatty acids. Inflammation. 2001; 25(1):17-23. DOI: 10.1023/a:1007019510569. View

20.

Curnutte J, Badwey J, Robinson J, Karnovsky M, Karnovsky M . Studies on the mechanism of superoxide release from human neutrophils stimulated with arachidonate. J Biol Chem. 1984; 259(19):11851-7. View