Bacterial ApbC Can Bind and Effectively Transfer Iron-sulfur Clusters

Overview

Journal Biochemistry

Specialty Biochemistry

Date 2008 Jul 12

PMID 18616280

Citations 28

Authors

Jeffrey M Boyd

Antonio J Pierik

Daili J A Netz

Roland Lill

Diana M Downs

Affiliations

Soon will be listed here.

Abstract

The metabolism of iron-sulfur ([Fe-S]) clusters requires a complex set of machinery that is still being defined. Mutants of Salmonella enterica lacking apbC have nutritional and biochemical properties indicative of defects in [Fe-S] cluster metabolism. ApbC is a 40.8 kDa homodimeric ATPase and as purified contains little iron and no acid-labile sulfide. An [Fe-S] cluster was reconstituted on ApbC, generating a protein that bound 2 mol of Fe and 2 mol of S (2-) per ApbC monomer and had a UV-visible absorption spectrum similar to known [4Fe-4S] cluster proteins. Holo-ApbC could rapidly and effectively activate Saccharomyces cerevisiae apo-isopropylmalate isolomerase (Leu1) in vitro, a process known to require the transfer of a [4Fe-4S] cluster. Maximum activation was achieved with 2 mol of ApbC per 1 mol of apo-Leu1. This article describes the first biochemical activity of ApbC in the context of [Fe-S] cluster metabolism. The data herein support a model in which ApbC coordinates an [4Fe-4S] cluster across its dimer interface and can transfer this cluster to an apoprotein acting as an [Fe-S] cluster scaffold protein, a function recently deduced for its eukaryotic homologues.

Citing Articles

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Esquilin-Lebron K, Dubrac S, Barras F, Boyd J mBio. 2021; 12(6):e0242521.

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A two-hybrid system reveals previously uncharacterized protein-protein interactions within the Helicobacter pylori NIF iron-sulfur maturation system.

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Iron-Sulfur Cluster Biogenesis and Iron Homeostasis in Cyanobacteria.

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The bacterial Mrp is a novel Mrp/NBP35 protein involved in iron-sulfur biogenesis.

Pardoux R, Fievet A, Carreira C, Brochier-Armanet C, Valette O, Dermoun Z Sci Rep. 2019; 9(1):712.

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Function and crystal structure of the dimeric P-loop ATPase CFD1 coordinating an exposed [4Fe-4S] cluster for transfer to apoproteins.

Stehling O, Jeoung J, Freibert S, Paul V, Banfer S, Niggemeyer B Proc Natl Acad Sci U S A. 2018; 115(39):E9085-E9094.

PMID: 30201724 PMC: 6166825. DOI: 10.1073/pnas.1807762115.

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