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Alta-Cyclic: a Self-optimizing Base Caller for Next-generation Sequencing

Overview
Journal Nat Methods
Specialties Biomedical Engineering
Pathology
Date 2008 Jul 8
PMID 18604217
Citations 88
Authors
Yaniv Erlich
Partha P Mitra
Melissa DelaBastide
W Richard McCombie
Gregory J Hannon
Affiliations
Soon will be listed here.
Abstract

Next-generation sequencing is limited to short read lengths and by high error rates. We systematically analyzed sources of noise in the Illumina Genome Analyzer that contribute to these high error rates and developed a base caller, Alta-Cyclic, that uses machine learning to compensate for noise factors. Alta-Cyclic substantially improved the number of accurate reads for sequencing runs up to 78 bases and reduced systematic biases, facilitating confident identification of sequence variants.

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