Denaturing Gradient Gel Electrophoresis As a Tool to Determine Batch Similarity of Probiotic Cultures of Porcine Cecal Bacteria

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2008 Jul 1

PMID 18586972

Citations 1

Authors

Michael E Hume

Charles M Scanlan

Roger B Harvey

Kathleen Andrews

James D Snodgrass

Armen G Nalian

Alexandra Martynova-Van Kley

David J Nisbet

Affiliations

Soon will be listed here.

Abstract

A continuous-flow porcine cecal bacterial culture has been used experimentally as treatment against enterotoxigenic Escherichia coli in weanling pigs. Periodically, the cultures must be started from frozen stock. Our results indicate that denaturing gradient gel electrophoresis can be applied as an indirect indication of culture similarity for each new batch generated from frozen stock.

Citing Articles

Establishment and characterization of a competitive exclusion bacterial culture derived from Nile tilapia (Oreochromis niloticus) gut microbiomes showing antibacterial activity against pathogenic Streptococcus agalactiae.

Melo-Bolivar J, Ruiz Pardo R, Hume M, Nisbet D, Rodriguez-Villamizar F, Alzate J PLoS One. 2019; 14(5):e0215375.

PMID: 31050668 PMC: 6499431. DOI: 10.1371/journal.pone.0215375.

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