Rapid Induction of Pluripotency Genes After Exposure of Human Somatic Cells to Mouse ES Cell Extracts

Overview

Journal Exp Cell Res

Specialty Cell Biology

Date 2008 Jun 24

PMID 18571647

Citations 29

Authors

Thierry Bru

Catriona Clarke

Michael J McGrew

Helen M Sang

Ian Wilmut

J Julian Blow

Affiliations

Soon will be listed here.

Abstract

The expression of 4 pluripotency genes (Oct4, Sox2, c-Myc and Klf4) in mouse embryonic fibroblasts can reprogramme them to a pluripotent state. We have investigated the expression of these pluripotency genes when human somatic 293T cells are permeabilized and incubated in extracts of mouse embryonic stem (ES) cells. Expression of all 4 genes was induced over 1-8 h. Gene expression was associated with loss of repressive histone H3 modifications and increased recruitment of RNA polymerase II at the promoters. Lamin A/C, which is typically found only in differentiated cells, was also removed from the nuclei. When 293T cells were returned to culture after exposure to ES cell extract, the expression of the pluripotency genes continued to rise over the following 48 h of culture, suggesting that long-term reprogramming of gene expression had been induced. This provides a methodology for studying the de-differentiation of somatic cells that can potentially lead to an efficient way of reprogramming somatic cells to a pluripotent state without genetically altering them.

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