Gene Expression Profile of Hemocytes of Kuruma Shrimp, Marsupenaeus Japonicus Following Peptidoglycan Stimulation

Overview

Journal Mar Biotechnol (NY)

Specialties Biology
Biotechnology

Date 2008 Jun 20

PMID 18563487

Citations 9

Authors

Fernand F Fagutao

Motoshige Yasuike

Christopher Marlowe Caipang

Hidehiro Kondo

Ikuo Hirono

Yukinori Takahashi

Takashi Aoki

Affiliations

Soon will be listed here.

Abstract

Shrimps are believed to lack an adaptive immune system and therefore rely heavily on their innate immune mechanisms to ward off pathogens. Moreover, their innate defense reactions are triggered by bacterial and fungal cell wall components such as lipopolysaccharides, peptidoglycan and beta-glucans. In this study, we used microarray to examine the gene expression profile of kuruma shrimp, Marsupenaeus japonicus, after stimulation with peptidoglycan. Subsequent results show that the number of upregulated genes and percentage of differential expression (21%) was highest at day 1 poststimulation. Differentially expressed genes in day 7 and day 14, on the other hand, were 3.25% and 11.21%, respectively. Sixty-one (61) genes of unknown function were found to have responded outright to peptidoglycan (PG) stimulation. Administration of PG also caused increases in the expressions of crustin, lysozyme, and a few antibacterial peptides, all of which are known to be involved in crustacean immune response. Taken together, our results suggest that innate response in shrimp is triggered instantaneously upon exposure to a bacterial component.

Citing Articles

Microarray Analysis of Immunity Against WSSV in Response to Injection of Non-specific Long dsRNA in Kuruma Shrimp, Marsupenaeus japonicus.

Maralit B, Komatsu M, Hipolito S, Hirono I, Kondo H Mar Biotechnol (NY). 2015; 17(4):493-501.

PMID: 25953417 DOI: 10.1007/s10126-015-9637-9.

QTL for white spot syndrome virus resistance and the sex-determining locus in the Indian black tiger shrimp (Penaeus monodon).

Robinson N, Gopikrishna G, Baranski M, Katneni V, Shekhar M, Shanmugakarthik J BMC Genomics. 2014; 15:731.

PMID: 25164406 PMC: 4158065. DOI: 10.1186/1471-2164-15-731.

Enhancement of shrimp antiviral immune response through caspase-dependent apoptosis by small molecules.

Zhi B, Tang W, Zhang X Mar Biotechnol (NY). 2010; 13(3):575-83.

PMID: 20936319 DOI: 10.1007/s10126-010-9328-5.

A gC1qR prevents white spot syndrome virus replication in the freshwater crayfish Pacifastacus leniusculus.

Watthanasurorot A, Jiravanichpaisal P, Soderhall I, Soderhall K J Virol. 2010; 84(20):10844-51.

PMID: 20686021 PMC: 2950596. DOI: 10.1128/JVI.01045-10.

Microarray analyses of shrimp immune responses.

Aoki T, Wang H, Unajak S, Santos M, Kondo H, Hirono I Mar Biotechnol (NY). 2010; 13(4):629-38.

PMID: 20393773 DOI: 10.1007/s10126-010-9291-1.

References

Citarasu T, Sivaram V, Immanuel G, Rout N, Murugan V . Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes. Fish Shellfish Immunol. 2006; 21(4):372-84. DOI: 10.1016/j.fsi.2006.01.002. View

Darias M, Zambonino-Infante J, Hugot K, Cahu C, Mazurais D . Gene expression patterns during the larval development of European sea bass (dicentrarchus labrax) by microarray analysis. Mar Biotechnol (NY). 2008; 10(4):416-28. DOI: 10.1007/s10126-007-9078-1. View

Moriya S, Sato S, Azumaya T, Suzuki O, Urawa S, Urano A . Genetic stock identification of chum salmon in the Bering Sea and North Pacific Ocean using mitochondrial DNA microarray. Mar Biotechnol (NY). 2006; 9(2):179-91. DOI: 10.1007/s10126-006-6079-4. View

Tincu J, Taylor S . Antimicrobial peptides from marine invertebrates. Antimicrob Agents Chemother. 2004; 48(10):3645-54. PMC: 521913. DOI: 10.1128/AAC.48.10.3645-3654.2004. View

Yasuike M, Kondo H, Hirono I, Aoki T . Difference in Japanese flounder, Paralichthys olivaceus gene expression profile following hirame rhabdovirus (HIRRV) G and N protein DNA vaccination. Fish Shellfish Immunol. 2007; 23(3):531-41. DOI: 10.1016/j.fsi.2006.12.006. View

Misra C, Das B, Pradhan J, Pattnaik P, Sethi S, Mukherjee S . Changes in lysosomal enzyme activity and protection against Vibrio infection in Macrobrachium rosenbergii (De Man) post larvae after bath immunostimulation with beta-glucan. Fish Shellfish Immunol. 2004; 17(4):389-95. DOI: 10.1016/j.fsi.2004.04.008. View

Tsai J, Wang H, Leu J, Hsiao H, Wang A, Kou G . Genomic and proteomic analysis of thirty-nine structural proteins of shrimp white spot syndrome virus. J Virol. 2004; 78(20):11360-70. PMC: 521807. DOI: 10.1128/JVI.78.20.11360-11370.2004. View

Smith V, Brown J, Hauton C . Immunostimulation in crustaceans: does it really protect against infection?. Fish Shellfish Immunol. 2003; 15(1):71-90. DOI: 10.1016/s1050-4648(02)00140-7. View

Kurobe T, Yasuike M, Kimura T, Hirono I, Aoki T . Expression profiling of immune-related genes from Japanese flounder Paralichthys olivaceus kidney cells using cDNA microarrays. Dev Comp Immunol. 2005; 29(6):515-23. DOI: 10.1016/j.dci.2004.10.005. View

10.

Chang C, Su M, Chen H, Liao I . Dietary beta-1,3-glucan effectively improves immunity and survival of Penaeus monodon challenged with white spot syndrome virus. Fish Shellfish Immunol. 2003; 15(4):297-310. DOI: 10.1016/s1050-4648(02)00167-5. View

11.

Peng X, Wood C, Blalock E, Chen K, Landfield P, Stromberg A . Statistical implications of pooling RNA samples for microarray experiments. BMC Bioinformatics. 2003; 4:26. PMC: 166151. DOI: 10.1186/1471-2105-4-26. View

12.

Khadijah S, Neo S, Hossain M, Miller L, Mathavan S, Kwang J . Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray. J Virol. 2003; 77(18):10162-7. PMC: 224607. DOI: 10.1128/jvi.77.18.10162-10167.2003. View

13.

de Hoon M, Imoto S, Nolan J, Miyano S . Open source clustering software. Bioinformatics. 2004; 20(9):1453-4. DOI: 10.1093/bioinformatics/bth078. View

14.

Okumura T . Effects of lipopolysaccharide on gene expression of antimicrobial peptides (penaeidins and crustin), serine proteinase and prophenoloxidase in haemocytes of the Pacific white shrimp, Litopenaeus vannamei. Fish Shellfish Immunol. 2006; 22(1-2):68-76. DOI: 10.1016/j.fsi.2006.03.013. View

15.

Chang C, Chen H, Su M, Liao I . Immunomodulation by dietary beta-1, 3-glucan in the brooders of the black tiger shrimp Penaeus monodon. Fish Shellfish Immunol. 2000; 10(6):505-14. DOI: 10.1006/fsim.2000.0266. View

16.

Song Y, Liu J, Chan L, Sung H . Glucan-induced disease resistance in tiger shrimp (Penaeus monodon). Dev Biol Stand. 1997; 90:413-21. View

17.

Kochzius M, Nolte M, Weber H, Silkenbeumer N, Hjorleifsdottir S, Hreggvidsson G . DNA microarrays for identifying fishes. Mar Biotechnol (NY). 2008; 10(2):207-17. PMC: 2263118. DOI: 10.1007/s10126-007-9068-3. View

18.

Steiner H . Peptidoglycan recognition proteins: on and off switches for innate immunity. Immunol Rev. 2004; 198:83-96. DOI: 10.1111/j.0105-2896.2004.0120.x. View

19.

Saldanha A . Java Treeview--extensible visualization of microarray data. Bioinformatics. 2004; 20(17):3246-8. DOI: 10.1093/bioinformatics/bth349. View

20.

Auslander M, Yudkovski Y, Chalifa-Caspi V, Herut B, Ophir R, Reinhardt R . Pollution-affected fish hepatic transcriptome and its expression patterns on exposure to cadmium. Mar Biotechnol (NY). 2008; 10(3):250-61. PMC: 2921062. DOI: 10.1007/s10126-007-9060-y. View