Detection of Hepatitis B Virus Pre-S1 and Pre-S2 Determinants in Paraffin Wax Embedded Liver Tissue: Importance of Reagents Used

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1991 Jul 1

PMID 1856286

Citations 1

Authors

N Hadzic

A Alberti

B Portmann

D Vergani

Affiliations

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Abstract

The presence of pre-S polypeptides in paraffin wax embedded liver sections from the biopsy specimens of 15 hepatitis B surface antigen (HBsAg) seropositive patients (five with chronic persistent hepatitis (CPH), four with chronic active hepatitis (CAH), four with cirrhosis and two "healthy" HBsAg carriers) was investigated using monoclonal antibodies directed to distinct epitopes on pre-S1 (18/7 and TO 606) and pre-S2 (5535 and Q 19/10). Pre-S1 was found in 13 cases when MA 18/7 was used but in only one specimen when TO 606 was used. Pre-S2 was detected in all the biopsy specimens with 5535 and in eight samples with Q 19/10. Mild enzymatic digestion annulled the staining of all monoclonal antibodies but Q 19/10. No association was observed between pre-S polypeptide expression and hepatitis B virus (HBV) replication or disease severity. Pre-S polypeptides can be detected readily in paraffin wax embedded material but the results obtained largely depend on the monoclonal antibodies used.

Citing Articles

Differential effect of chronic hepatitis D virus infection on intrahepatic expression of hepatitis B viral antigen.

Lau J, Portmann B, Alexander G, Williams R J Clin Pathol. 1992; 45(4):314-8.

PMID: 1577968 PMC: 495271. DOI: 10.1136/jcp.45.4.314.

References

Theilmann L, Klinkert M, Gmelin K, Salfeld J, Schaller H, Pfaff E . Detection of pre-S1 proteins in serum and liver of HBsAg-positive patients: a new marker for hepatitis B virus infection. Hepatology. 1986; 6(2):186-90. DOI: 10.1002/hep.1840060206. View

Hadziyannis S, Gerber M, Vissoulis C, Popper H . Cytoplasmic hepatitis B antigen in "ground-glass" hepatocytes of carriers. Arch Pathol. 1973; 96(5):327-30. View

Tiollais P, Pourcel C, Dejean A . The hepatitis B virus. Nature. 1985; 317(6037):489-95. DOI: 10.1038/317489a0. View

Fraiese A, Pontisso P, Cavalletto D, Fattovich G, Alberti A . Expression of preS1 and preS2 in the liver of chronic hepatitis B virus carriers. J Hepatol. 1988; 7(2):157-63. DOI: 10.1016/s0168-8278(88)80477-x. View

Gerber M, Thung S . The pre-S2 region of hepatitis B virus: more questions than answers. Hepatology. 1989; 9(2):328-30. DOI: 10.1002/hep.1840090227. View

Heermann K, Goldmann U, Schwartz W, Seyffarth T, Baumgarten H, Gerlich W . Large surface proteins of hepatitis B virus containing the pre-s sequence. J Virol. 1984; 52(2):396-402. PMC: 254539. DOI: 10.1128/JVI.52.2.396-402.1984. View

Hadziyannis S, Raimondo G, Papaioannou C, Anastassakos C, Wong D, Sninsky J . Expression of pre-S gene-encoded proteins in liver and serum during chronic hepatitis B virus infection in comparison to other markers of active virus replication. J Hepatol. 1987; 5(3):253-9. DOI: 10.1016/s0168-8278(87)80029-6. View

Gerber M, Thung S . Molecular and cellular pathology of hepatitis B. Lab Invest. 1985; 52(6):572-90. View

Thung S, Gerber M . HBsAg-associated albumin receptors and antialbumin antibodies in sera of patients with liver disease. Gastroenterology. 1981; 80(2):260-4. View

10.

Vergani D, Locasciulli A, Masera G, Alberti A, Moroni G, TEE D . Histological evidence of hepatitis-B-virus infection with negative serology in children with acute leukaemia who develop chronic liver disease. Lancet. 1982; 1(8268):361-4. DOI: 10.1016/s0140-6736(82)91392-7. View

11.

Imai M, Yanase Y, Nojiri T, Miyakawa Y, Mayumi M . A receptor for polymerized human and chimpanzee albumins on hepatitis B virus particles co-occurring with HBeAg. Gastroenterology. 1979; 76(2):242-7. View

12.

Thomsen P, POULSEN H, Petersen P . Different types of ground glass hepatocytes in human liver biopsies: morphology, occurrence and diagnostic significance. Scand J Gastroenterol. 1976; 11(2):113-9. View

13.

Deodhar K, TAPP E, Scheuer P . Orcein staining of hepatitis B antigen in paraffin sections of liver biopsies. J Clin Pathol. 1975; 28(1):66-70. PMC: 475596. DOI: 10.1136/jcp.28.1.66. View

14.

WINCKLER K, Junge U, Creutzfeldt W . Ground-glass hepatocytes in unselected liver biopsies. ultrastructure and relationship to hepatitis B surface antigen. Scand J Gastroenterol. 1976; 11(2):167-70. View

15.

Portmann B, Galbraith R, Eddleston A, Zuckerman A, Williams R . Detection of HBSAG in fixed liver tissue - use of a modified immunofluorescent technique and comparison with histochemical methods. Gut. 1976; 17(1):1-9. PMC: 1411055. DOI: 10.1136/gut.17.1.1. View

16.

Neurath A, Kent S, Strick N, Parker K . Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell. 1986; 46(3):429-36. DOI: 10.1016/0092-8674(86)90663-x. View