Use of Fluorescence in Situ Hybridization for Rapid Identification of Staphylococci in Blood Culture Samples Collected in a Portuguese Hospital

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2008 Jun 20

PMID 18562589

Citations 4

Authors

Ana Tavares

Joao Inacio

Jose Melo-Cristino

Isabel Couto

Affiliations

Soon will be listed here.

Abstract

Fluorescence in situ hybridization was used for the direct identification of staphylococci in blood cultures collected at a Portuguese hospital where staphylococci account for up to 35% of clinically relevant blood cultures. The assay was able to detect the presence/absence of staphylococci and distinguish Staphylococcus aureus from coagulase-negative staphylococci in 4.5 h.

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References

Kempf V, Trebesius K, Autenrieth I . Fluorescent In situ hybridization allows rapid identification of microorganisms in blood cultures. J Clin Microbiol. 2000; 38(2):830-8. PMC: 86216. DOI: 10.1128/JCM.38.2.830-838.2000. View

Chapin K, Musgnug M . Evaluation of three rapid methods for the direct identification of Staphylococcus aureus from positive blood cultures. J Clin Microbiol. 2003; 41(9):4324-7. PMC: 193828. DOI: 10.1128/JCM.41.9.4324-4327.2003. View

Biedenbach D, Moet G, Jones R . Occurrence and antimicrobial resistance pattern comparisons among bloodstream infection isolates from the SENTRY Antimicrobial Surveillance Program (1997-2002). Diagn Microbiol Infect Dis. 2004; 50(1):59-69. DOI: 10.1016/j.diagmicrobio.2004.05.003. View

Fuchs B, Wallner G, Beisker W, Schwippl I, Ludwig W, Amann R . Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes. Appl Environ Microbiol. 1998; 64(12):4973-82. PMC: 90951. DOI: 10.1128/AEM.64.12.4973-4982.1998. View

Hall K, Lyman J . Updated review of blood culture contamination. Clin Microbiol Rev. 2006; 19(4):788-802. PMC: 1592696. DOI: 10.1128/CMR.00062-05. View

Krimmer V, Merkert H, von Eiff C, Frosch M, Eulert J, Lohr J . Detection of Staphylococcus aureus and Staphylococcus epidermidis in clinical samples by 16S rRNA-directed in situ hybridization. J Clin Microbiol. 1999; 37(8):2667-73. PMC: 85309. DOI: 10.1128/JCM.37.8.2667-2673.1999. View

Wisplinghoff H, Bischoff T, Tallent S, Seifert H, Wenzel R, Edmond M . Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis. 2004; 39(3):309-17. DOI: 10.1086/421946. View

Peters R, Savelkoul P, Simoons-Smit A, Danner S, Vandenbroucke-Grauls C, van Agtmael M . Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice. J Clin Microbiol. 2006; 44(1):119-23. PMC: 1351964. DOI: 10.1128/JCM.44.1.119-123.2006. View

Miragaia M, Couto I, Pereira S, Kristinsson K, Westh H, Jarlov J . Molecular characterization of methicillin-resistant Staphylococcus epidermidis clones: evidence of geographic dissemination. J Clin Microbiol. 2002; 40(2):430-8. PMC: 153385. DOI: 10.1128/JCM.40.2.430-438.2002. View

10.

Hogardt M, Trebesius K, Geiger A, Hornef M, Rosenecker J, Heesemann J . Specific and rapid detection by fluorescent in situ hybridization of bacteria in clinical samples obtained from cystic fibrosis patients. J Clin Microbiol. 2000; 38(2):818-25. PMC: 86213. DOI: 10.1128/JCM.38.2.818-825.2000. View

11.

Gonzalez V, Padilla E, Gimenez M, Vilaplana C, Perez A, Fernandez G . Rapid diagnosis of Staphylococcus aureus bacteremia using S. aureus PNA FISH. Eur J Clin Microbiol Infect Dis. 2004; 23(5):396-8. DOI: 10.1007/s10096-004-1112-x. View

12.

Sanches I, Aires de Sousa M, Sobral L, Calheiros I, Felicio L, Pedra I . Multidrug-resistant Iberian epidemic clone of methicillin-resistant Staphylococcus aureus endemic in a hospital in northern Portugal. Microb Drug Resist. 1995; 1(4):299-306. DOI: 10.1089/mdr.1995.1.299. View

13.

Forrest G, Mehta S, Weekes E, Lincalis D, Johnson J, Venezia R . Impact of rapid in situ hybridization testing on coagulase-negative staphylococci positive blood cultures. J Antimicrob Chemother. 2006; 58(1):154-8. DOI: 10.1093/jac/dkl146. View

14.

Sanches I, Mato R, de Lencastre H, Tomasz A . Patterns of multidrug resistance among methicillin-resistant hospital isolates of coagulase-positive and coagulase-negative staphylococci collected in the international multicenter study RESIST in 1997 and 1998. Microb Drug Resist. 2001; 6(3):199-211. DOI: 10.1089/mdr.2000.6.199. View

15.

Amann R, Krumholz L, Stahl D . Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol. 1990; 172(2):762-70. PMC: 208504. DOI: 10.1128/jb.172.2.762-770.1990. View

16.

Oliveira K, Brecher S, Durbin A, Shapiro D, Schwartz D, De Girolami P . Direct identification of Staphylococcus aureus from positive blood culture bottles. J Clin Microbiol. 2003; 41(2):889-91. PMC: 149670. DOI: 10.1128/JCM.41.2.889-891.2003. View

17.

Brosius J, Dull T, Sleeter D, Noller H . Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol. 1981; 148(2):107-27. DOI: 10.1016/0022-2836(81)90508-8. View