Cloning and Sequence Analysis of the Major Outer Membrane Protein Gene of Chlamydia Psittaci 6BC

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1991 Aug 1

PMID 1856001

Citations 11

Authors

K D Everett

A A ANDERSEN

M Plaunt

T P Hatch

Affiliations

Soon will be listed here.

Abstract

The gene encoding the major outer membrane protein (MOMP) of the psittacine Chlamydia psittaci strain 6BC was cloned and sequenced. N-terminal protein sequencing of the mature MOMP indicated that it is posttranslationally processed at a site identical to the site previously identified in the MOMP of Chlamydia trachomatis L2. The nucleotide sequence of the C. psittaci 6BC MOMP gene was found to be 67 to 68% identical to those of human C. trachomatis strains, 73% identical to that of Chlamydia pneumoniae IOL-207, 79% identical to that of the C. psittaci guinea pig inclusion conjunctivitis strain, GPIC, and 83% identical to that of the C. psittaci ovine abortion strain S26/3. In contrast, the 6BC sequence was found to be greater than 99% identical to the sequences reported for two strains of C. psittaci, A22/M and Cal-10 meningopneumonitis, believed to be of nonpsittacine avian origin. Monoclonal antibody analysis confirmed the nonpsittacine avian origin of A22/M but identified the Cal-10 strain from which the MOMP gene was previously sequenced as a psittacine strain. These results confirm that psittacine and nonpsittacine avian strains of C. psittaci are closely related and distinct from the mammalian guinea pig inclusion conjunctivitis and ovine abortion strains of C. psittaci.

Citing Articles

The hypothetical protein CT813 is localized in the Chlamydia trachomatis inclusion membrane and is immunogenic in women urogenitally infected with C. trachomatis.

Chen C, Chen D, Sharma J, Cheng W, Zhong Y, Liu K Infect Immun. 2006; 74(8):4826-40.

PMID: 16861671 PMC: 1539634. DOI: 10.1128/IAI.00081-06.

Chlamydia-infected cells continue to undergo mitosis and resist induction of apoptosis.

Greene W, Xiao Y, Huang Y, McClarty G, Zhong G Infect Immun. 2003; 72(1):451-60.

PMID: 14688126 PMC: 343958. DOI: 10.1128/IAI.72.1.451-460.2004.

Recombination in the ompA gene but not the omcB gene of Chlamydia contributes to serovar-specific differences in tissue tropism, immune surveillance, and persistence of the organism.

Millman K, Tavare S, Dean D J Bacteriol. 2001; 183(20):5997-6008.

PMID: 11567000 PMC: 99679. DOI: 10.1128/JB.183.20.5997-6008.2001.

Chlamydophila abortus in a Brown skua (Catharacta antarctica lonnbergi) from a subantarctic island.

Herrmann B, Rahman R, Bergstrom S, Bonnedahl J, Olsen B Appl Environ Microbiol. 2000; 66(8):3654-6.

PMID: 10919838 PMC: 92202. DOI: 10.1128/AEM.66.8.3654-3656.2000.

High-level expression of Chlamydia psittaci major outer membrane protein in COS cells and in skeletal muscles of turkeys.

Vanrompay D, Cox E, Mast J, Goddeeris B, Volckaert G Infect Immun. 1998; 66(11):5494-500.

PMID: 9784562 PMC: 108688. DOI: 10.1128/IAI.66.11.5494-5500.1998.

References

GORDON F, QUAN A . OCCURENCE OF GLYCOGEN IN INCLUSIONS OF THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM-TRACHOMA AGENTS. J Infect Dis. 1965; 115:186-96. DOI: 10.1093/infdis/115.2.186. View

Hatch T, Vance Jr D . Identification of a major envelope protein in Chlamydia spp. J Bacteriol. 1981; 146(1):426-9. PMC: 217103. DOI: 10.1128/jb.146.1.426-429.1981. View

MURRAY E . GUINEA PIG INCLUSION CONJUNCTIVITIS VIRUS. I. ISOLATION AND IDENTIFICATION AS A MEMBER OF THE PSITTACOSIS-LYMPHOGRANULOMA-TRACHOMA GROUP. J Infect Dis. 1964; 114:1-12. DOI: 10.1093/infdis/114.1.1. View

ANDERSEN A . Serotyping of Chlamydia psittaci isolates using serovar-specific monoclonal antibodies with the microimmunofluorescence test. J Clin Microbiol. 1991; 29(4):707-11. PMC: 269857. DOI: 10.1128/jcm.29.4.707-711.1991. View

ANDERSEN A . Comparison of avian Chlamydia psittaci isolates by restriction endonuclease analysis and serovar-specific monoclonal antibodies. J Clin Microbiol. 1991; 29(2):244-9. PMC: 269747. DOI: 10.1128/jcm.29.2.244-249.1991. View

Carter M, Giles I, TREHARNE J, Ward M, Clark I . Nucleotide sequence and taxonomic value of the major outer membrane protein gene of Chlamydia pneumoniae IOL-207. J Gen Microbiol. 1991; 137(3):465-75. DOI: 10.1099/00221287-137-3-465. View

Zhang Y, Morrison S, Caldwell H, Baehr W . Cloning and sequence analysis of the major outer membrane protein genes of two Chlamydia psittaci strains. Infect Immun. 1989; 57(5):1621-5. PMC: 313324. DOI: 10.1128/iai.57.5.1621-1625.1989. View

McClenaghan M, Honeycombe J, Bevan B, Herring A . Distribution of plasmid sequences in avian and mammalian strains of Chlamydia psittaci. J Gen Microbiol. 1988; 134(3):559-65. DOI: 10.1099/00221287-134-3-559. View

Herring A, Tan T, Baxter S, Inglis N, DUNBAR S . Sequence analysis of the major outer membrane protein gene of an ovine abortion strain of Chlamydia psittaci. FEMS Microbiol Lett. 1989; 53(1-2):153-8. DOI: 10.1016/0378-1097(89)90383-2. View

10.

Stephens R, Wagar E, Inouye C, Urdea M . Diversity of Chlamydia trachomatis major outer membrane protein genes. J Bacteriol. 1987; 169(9):3879-85. PMC: 213681. DOI: 10.1128/jb.169.9.3879-3885.1987. View

11.

Conlan J, Clarke I, Ward M . Epitope mapping with solid-phase peptides: identification of type-, subspecies-, species- and genus-reactive antibody binding domains on the major outer membrane protein of Chlamydia trachomatis. Mol Microbiol. 1988; 2(5):673-9. DOI: 10.1111/j.1365-2958.1988.tb00076.x. View

12.

Baehr W, Zhang Y, Joseph T, Su H, Nano F, Everett K . Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes. Proc Natl Acad Sci U S A. 1988; 85(11):4000-4. PMC: 280348. DOI: 10.1073/pnas.85.11.4000. View

13.

Newhall 5th W, Terho P, Wilde 3rd C, Batteiger B, Jones R . Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies. J Clin Microbiol. 1986; 23(2):333-8. PMC: 268637. DOI: 10.1128/jcm.23.2.333-338.1986. View

14.

Batteiger B, Newhall 5th W, Terho P, Wilde 3rd C, Jones R . Antigenic analysis of the major outer membrane protein of Chlamydia trachomatis with murine monoclonal antibodies. Infect Immun. 1986; 53(3):530-3. PMC: 260822. DOI: 10.1128/iai.53.3.530-533.1986. View

15.

Nano F, Barstad P, Mayer L, Coligan J, Caldwell H . Partial amino acid sequence and molecular cloning of the encoding gene for the major outer membrane protein of Chlamydia trachomatis. Infect Immun. 1985; 48(2):372-7. PMC: 261316. DOI: 10.1128/iai.48.2.372-377.1985. View

16.

Zhang Y, Stewart S, Joseph T, Taylor H, Caldwell H . Protective monoclonal antibodies recognize epitopes located on the major outer membrane protein of Chlamydia trachomatis. J Immunol. 1987; 138(2):575-81. View

17.

BARNES R, Wang S, Kuo C, Stamm W . Rapid immunotyping of Chlamydia trachomatis with monoclonal antibodies in a solid-phase enzyme immunoassay. J Clin Microbiol. 1985; 22(4):609-13. PMC: 268477. DOI: 10.1128/jcm.22.4.609-613.1985. View

18.

Wang S, Kuo C, BARNES R, Stephens R, GRAYSTON J . Immunotyping of Chlamydia trachomatis with monoclonal antibodies. J Infect Dis. 1985; 152(4):791-800. DOI: 10.1093/infdis/152.4.791. View

19.

Tan T, Herring A, Anderson I, Jones G . Protection of sheep against Chlamydia psittaci infection with a subcellular vaccine containing the major outer membrane protein. Infect Immun. 1990; 58(9):3101-8. PMC: 313617. DOI: 10.1128/iai.58.9.3101-3108.1990. View

20.

Crenshaw R, Fahr M, Wichlan D, Hatch T . Developmental cycle-specific host-free RNA synthesis in Chlamydia spp. Infect Immun. 1990; 58(10):3194-201. PMC: 313639. DOI: 10.1128/iai.58.10.3194-3201.1990. View