Silkworm and Spider Silk Scaffolds for Chondrocyte Support

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2008 Jun 12

PMID 18545943

Citations 30

Authors

Kris Gellynck

Peter C M Verdonk

Els Van Nimmen

Karl F Almqvist

Tom Gheysens

Gustaaf Schoukens

Lieva Van Langenhove

Paul Kiekens

Johan Mertens

Gust Verbruggen

Affiliations

Soon will be listed here.

Abstract

Objective: To create scaffolds with silkworm cocoon, spider egg sac and spider dragline silk fibres and examine their use for chondrocyte attachment and support.

Methods: Three different kinds of scaffolds were developed with Bombyx mori cocoon, Araneus diadematus egg sac and dragline silk fibres. The attachment of human articular cartilage cells were investigated on these bioprotein matrices. The chondrocytes produced an extracellular matrix which was studied by immunostaining. Moreover, the compression behaviour in relation to the porosity was studied.

Results: The compression modulus of a silkworm silk scaffold was related to its porosity. Chondrocytes were able to attach and to grow on the different fibres and in the scaffolds for several weeks while producing extracellular matrix products.

Conclusion: Porous scaffolds can be made out of silkworm and spider silk for cartilage regeneration. Mechanical properties are related to porosity and pore size of the construct. Cell spreading and cell expression depended on the porosity and pore-size.

Citing Articles

Bacteria inhabiting spider webs enhance host silk extensibility.

Tsiareshyna M, Wang T, Lin Y, Piorkowski D, Huang S, Huang Y Sci Rep. 2024; 14(1):11011.

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Interfacial interactions between spider silk protein and cellulose studied by molecular dynamics simulation.

Zhao T, Ma H, Liu Y, Chen Z, Shi Q, Ning L J Mol Model. 2024; 30(5):156.

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Microstructure of the silk fibroin-based hydrogel scaffolds derived from the orb-web spider Trichonephila clavata.

Sun Y, Ku B, Moon M Appl Microsc. 2024; 54(1):3.

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Development of a More Environmentally Friendly Silk Fibroin Scaffold for Soft Tissue Applications.

Roblin N, DeBari M, Shefter S, Iizuka E, Abbott R J Funct Biomater. 2023; 14(4).

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Ning C, Li P, Gao C, Fu L, Liao Z, Tian G Front Bioeng Biotechnol. 2023; 11:1115312.

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