Genetic Modifier Screens Reveal New Components That Interact with the Drosophila Dystroglycan-dystrophin Complex

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2008 Jun 12

PMID 18545683

Citations 42

Authors

Mariya M Kucherenko

Mario Pantoja

Andriy S Yatsenko

Halyna R Shcherbata

Karin A Fischer

Dariya V Maksymiv

Yaroslava I Chernyk

Hannele Ruohola-Baker

Affiliations

Soon will be listed here.

Abstract

The Dystroglycan-Dystrophin (Dg-Dys) complex has a capacity to transmit information from the extracellular matrix to the cytoskeleton inside the cell. It is proposed that this interaction is under tight regulation; however the signaling/regulatory components of Dg-Dys complex remain elusive. Understanding the regulation of the complex is critical since defects in this complex cause muscular dystrophy in humans. To reveal new regulators of the Dg-Dys complex, we used a model organism Drosophila melanogaster and performed genetic interaction screens to identify modifiers of Dg and Dys mutants in Drosophila wing veins. These mutant screens revealed that the Dg-Dys complex interacts with genes involved in muscle function and components of Notch, TGF-beta and EGFR signaling pathways. In addition, components of pathways that are required for cellular and/or axonal migration through cytoskeletal regulation, such as Semaphorin-Plexin, Frazzled-Netrin and Slit-Robo pathways show interactions with Dys and/or Dg. These data suggest that the Dg-Dys complex and the other pathways regulating extracellular information transfer to the cytoskeletal dynamics are more intercalated than previously thought.

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