Programmatic Implementation of Rapid DST for Mycobacterium Tuberculosis in Peru

Overview

Journal Int J Tuberc Lung Dis

Specialty Pulmonary Medicine

Date 2008 Jun 12

PMID 18544198

Citations 7

Authors

L Asencios

G Yale

M Yagui

N Quispe

A Taylor

J Blaya

C Contreras

P Cegielski

J Bayona

C Bonilla

S Shin

Affiliations

Soon will be listed here.

Abstract

Background: Performance characteristics of novel rapid drug susceptibility tests (DST) for Mycobacterium tuberculosis may change when moving from research to implementation in actual public health practice. We describe the performance characteristics of a direct, rapid DST when implemented in Lima, Peru.

Methods: A district laboratory validated conventional proportions and nitrate reductase methods. We collected data on samples submitted for DST from January 2005 to June 2007 and calculated frequency of testing and results, and median time to test results.

Results: A total of 4102 DSTs were performed by conventional DST and 895 by nitrate reductase. Results were obtained from 72.8% of samples by conventional DST and from 70.2% of those processed by Griess; respectively 26.4% and 31.5% were multidrug-resistant tuberculosis. The median time from sample collection to test result was 31 days for Griess vs. 99 days for conventional DST.

Conclusions: Preliminary experience with the Griess method demonstrates favorable performance under program conditions.

Citing Articles

Impact of HIV on mortality among patients treated for tuberculosis in Lima, Peru: a prospective cohort study.

Velasquez G, Cegielski J, Murray M, Yagui M, Asencios L, Bayona J BMC Infect Dis. 2016; 16:45.

PMID: 26831140 PMC: 4736097. DOI: 10.1186/s12879-016-1375-8.

Nitrate reductase assay for rapid detection of isoniazid, rifampin, ethambutol, and streptomycin resistance in Mycobacterium tuberculosis: a systematic review and meta-analysis.

Coban A, Deveci A, Sunter A, Martin A J Clin Microbiol. 2013; 52(1):15-9.

PMID: 24131684 PMC: 3911439. DOI: 10.1128/JCM.01990-13.

Validation of microscopic observation drug susceptibility testing for rapid, direct rifampicin and isoniazid drug susceptibility testing in patients receiving tuberculosis treatment.

Coronel J, Roper M, Herrera C, Bonilla C, Jave O, Gianella C Clin Microbiol Infect. 2013; 20(6):536-41.

PMID: 24107197 PMC: 4302318. DOI: 10.1111/1469-0691.12401.

Impact of rapid drug susceptibility testing for tuberculosis: program experience in Lima, Peru.

Shin S, Asencios L, Yagui M, Yale G, Suarez C, Bayona J Int J Tuberc Lung Dis. 2012; 16(11):1538-43.

PMID: 22990138 PMC: 5788451. DOI: 10.5588/ijtld.12.0071.

Targeted drug-resistance testing strategy for multidrug-resistant tuberculosis detection, Lima, Peru, 2005-2008.

Velasquez G, Yagui M, Cegielski J, Asencios L, Bayona J, Bonilla C Emerg Infect Dis. 2011; 17(3):432-40.

PMID: 21392434 PMC: 3166030. DOI: 10.3201/eid1703.101553.

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