Alpha 2.0
Login Register
» Articles » PMID: 1851882

Efficient Polyadenylation Within the Human Immunodeficiency Virus Type 1 Long Terminal Repeat Requires Flanking U3-specific Sequences

Overview
Journal J Virol
Publisher American Society for Microbiology
Date 1991 Jun 1
PMID 1851882
Citations 51
Authors
P H Brown
L S Tiley
B R Cullen
Affiliations
Soon will be listed here.
Abstract

During transcription of the human immunodeficiency virus type 1 provirus, polyadenylation signals present in the 5' long terminal repeat (LTR) are disregarded while the identical polyadenylation signals present in the 3' LTR are utilized efficiently. As both transcribed LTR sequences contain all signals known to be required for efficient polyadenylation, the basis for this differential utilization has been unclear. Here, we describe experiments that suggest that transcribed sequences present within the human immunodeficiency virus type 1 LTR U3 region act in cis to enhance polyadenylation within the 3' LTR.

Citing Articles

Role of RNA structural plasticity in modulating HIV-1 genome packaging and translation.

Yasin S, Lesko S, Kharytonchyk S, Brown J, Chaudry I, Geleta S Proc Natl Acad Sci U S A. 2024; 121(33):e2407400121.

PMID: 39110735 PMC: 11331132. DOI: 10.1073/pnas.2407400121.

CAR-T cell potency: from structural elements to vector backbone components.

Mazinani M, Rahbarizadeh F Biomark Res. 2022; 10(1):70.

PMID: 36123710 PMC: 9487061. DOI: 10.1186/s40364-022-00417-w.

Cell Cycle Kinase Polo Is Controlled by a Widespread 3' Untranslated Region Regulatory Sequence in Drosophila melanogaster.

Oliveira M, Freitas J, Pinto P, de Jesus A, Tavares J, Pinho M Mol Cell Biol. 2019; 39(15).

PMID: 31085682 PMC: 6639250. DOI: 10.1128/MCB.00581-18.

Minor Contribution of Chimeric Host-HIV Readthrough Transcripts to the Level of HIV Cell-Associated gag RNA.

Pasternak A, DeMaster L, Kootstra N, Reiss P, ODoherty U, Berkhout B J Virol. 2015; 90(2):1148-51.

PMID: 26559833 PMC: 4702708. DOI: 10.1128/JVI.02597-15.

HSP70 binding protein 1 (HspBP1) suppresses HIV-1 replication by inhibiting NF-κB mediated activation of viral gene expression.

Chaudhary P, Khan S, Rawat P, Augustine T, Raynes D, Guerriero V Nucleic Acids Res. 2015; 44(4):1613-29.

PMID: 26538602 PMC: 4770212. DOI: 10.1093/nar/gkv1151.

References
1.
Valsamakis A, Zeichner S, Carswell S, Alwine J . The human immunodeficiency virus type 1 polyadenylylation signal: a 3' long terminal repeat element upstream of the AAUAAA necessary for efficient polyadenylylation. Proc Natl Acad Sci U S A. 1991; 88(6):2108-12. PMC: 51178. DOI: 10.1073/pnas.88.6.2108. View
2.
Iwasaki K, Temin H . The efficiency of RNA 3'-end formation is determined by the distance between the cap site and the poly(A) site in spleen necrosis virus. Genes Dev. 1990; 4(12B):2299-307. DOI: 10.1101/gad.4.12b.2299. View
3.
Carswell S, Alwine J . Efficiency of utilization of the simian virus 40 late polyadenylation site: effects of upstream sequences. Mol Cell Biol. 1989; 9(10):4248-58. PMC: 362504. DOI: 10.1128/mcb.9.10.4248-4258.1989. View
4.
Mullis K, Faloona F . Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987; 155:335-50. DOI: 10.1016/0076-6879(87)55023-6. View
5.
Ju G, Cullen B . The role of avian retroviral LTRs in the regulation of gene expression and viral replication. Adv Virus Res. 1985; 30:179-223. DOI: 10.1016/s0065-3527(08)60451-0. View