Molecular Characterization of Porcine Picobirnaviruses and Development of a Specific Reverse Transcription-PCR Assay

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2008 May 30

PMID 18508933

Citations 16

Authors

Gabriela M Carruyo

Guaniri Mateu

Laura C Martinez

Flor H Pujol

Silvia V Nates

Ferdinando Liprandi

Juan E Ludert

Affiliations

Soon will be listed here.

Abstract

The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.

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