Regulation of the Multiple Promoters of the Human Aldolase A Gene: Response of Its Two Ubiquitous Promoters to Agents Promoting Cell Proliferation

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1991 Feb 25

PMID 1850123

Citations 8

Authors

S Gautron

P Maire

V Hakim

A Kahn

Affiliations

Soon will be listed here.

Abstract

The human aldolase A gene is transcribed from three distinct promoters, the two ubiquitous promoters PN and PH and the muscle specific promoter PM. In the present study, we investigate further aldolase A mRNA structure and expression. We demonstrate that the upstream N-type exon is, in fact, extremely heterogeneous. RNAse H mapping experiments permit quantification of relative abundance of N, M, and H type mRNAs and show that the level of transcripts containing the downstream H-type exon is at least 30 times higher than that of those containing N exon, in all tissues tested. Aldolase A level is up-regulated in proliferating cells. Here we show that both N and H type mRNAs, although barely detectable in normal liver, are highly expressed in human hepatomas biopsies. Furthermore, in human lymphocytes, N-type mRNA level is enhanced by serum treatment, while in cultured Hep G2 cells, both N-type and H-type mRNA levels are increased by serum and by the tumor promoting agent PMA. Using CAT constructs in transfection experiments, we demonstrate that the H exon plus its upstream region can function autonomously: the 420 base pairs upstream of the H exon are sufficient to confer to promoter PH an efficiency comparable that of the complete SV40 early promoter and enhancer in two cell lines.

Citing Articles

The human pH aldolase A promoter directs widespread but muscle-predominant expression in transgenic mice.

Moch C, Spitz F, Porteu A, Kahn A, Daegelen D Transgenic Res. 1998; 7(2):113-21.

PMID: 9608739 DOI: 10.1023/a:1008820409079.

Characterization of the promoter of human adipocyte hormone-sensitive lipase.

GROBER J, Laurell H, Blaise R, Fabry B, Schaak S, Holm C Biochem J. 1998; 328 ( Pt 2):453-61.

PMID: 9371701 PMC: 1218941. DOI: 10.1042/bj3280453.

Purification of a dichlorophenol-indophenol oxidoreductase from rat and bovine synaptic membranes: tight complex association of a glyceraldehyde-3-phosphate dehydrogenase isoform, TOAD64, enolase-gamma and aldolase C.

Bulliard C, Zurbriggen R, Tornare J, Faty M, Dastoor Z, Dreyer J Biochem J. 1997; 324 ( Pt 2):555-63.

PMID: 9182718 PMC: 1218466. DOI: 10.1042/bj3240555.

Independence and interdependence of the three human aldolase A promoters in transgenic mice.

Moch C, Kahn A, Daegelen D Gene Expr. 1996; 6(1):1-14.

PMID: 8931987 PMC: 6148263.

An opportunistic promoter sharing regulatory sequences with either a muscle-specific or a ubiquitous promoter in the human aldolase A gene.

Concordet J, Salminen M, Demignon J, Moch C, Maire P, Kahn A Mol Cell Biol. 1993; 13(1):9-17.

PMID: 8417365 PMC: 358879. DOI: 10.1128/mcb.13.1.9-17.1993.

References

Matrisian L, Rautmann G, Magun B, Breathnach R . Epidermal growth factor or serum stimulation of rat fibroblasts induces an elevation in mRNA levels for lactate dehydrogenase and other glycolytic enzymes. Nucleic Acids Res. 1985; 13(3):711-26. PMC: 341030. DOI: 10.1093/nar/13.3.711. View

Chirgwin J, Przybyla A, MacDonald R, Rutter W . Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 1979; 18(24):5294-9. DOI: 10.1021/bi00591a005. View

Sehgal A, Patil N, Chao M . A constitutive promoter directs expression of the nerve growth factor receptor gene. Mol Cell Biol. 1988; 8(8):3160-7. PMC: 363544. DOI: 10.1128/mcb.8.8.3160-3167.1988. View

Ginot F, Decaux J, Cognet M, Berbar T, Levrat F, Kahn A . Transfection of hepatic genes into adult rat hepatocytes in primary culture and their tissue-specific expression. Eur J Biochem. 1989; 180(2):289-94. DOI: 10.1111/j.1432-1033.1989.tb14646.x. View

Izzo P, Costanzo P, Lupo A, Rippa E, Paolella G, Salvatore F . Human aldolase A gene. Structural organization and tissue-specific expression by multiple promoters and alternate mRNA processing. Eur J Biochem. 1988; 174(4):569-78. DOI: 10.1111/j.1432-1033.1988.tb14136.x. View

Lamas E, Kahn A, Guillouzo A . Detection of mRNAs present at low concentrations in rat liver by in situ hybridization: application to the study of metabolic regulation and azo dye hepatocarcinogenesis. J Histochem Cytochem. 1987; 35(5):559-63. DOI: 10.1177/35.5.3104450. View

DE Wet J, Wood K, DeLuca M, Helinski D, Subramani S . Firefly luciferase gene: structure and expression in mammalian cells. Mol Cell Biol. 1987; 7(2):725-37. PMC: 365129. DOI: 10.1128/mcb.7.2.725-737.1987. View

Imagawa M, Chiu R, Karin M . Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP. Cell. 1987; 51(2):251-60. DOI: 10.1016/0092-8674(87)90152-8. View

Joh K, Arai Y, Mukai T, Hori K . Expression of three mRNA species from a single rat aldolase A gene, differing in their 5' non-coding regions. J Mol Biol. 1986; 190(3):401-10. DOI: 10.1016/0022-2836(86)90011-2. View

10.

Gautron S, Daegelen D, Mennecier F, Dubocq D, Kahn A, DREYFUS J . Molecular mechanisms of McArdle's disease (muscle glycogen phosphorylase deficiency). RNA and DNA analysis. J Clin Invest. 1987; 79(1):275-81. PMC: 424040. DOI: 10.1172/JCI112794. View

11.

Maire P, Gautron S, Hakim V, Gregori C, Mennecier F, Kahn A . Characterization of three optional promoters in the 5' region of the human aldolase A gene. J Mol Biol. 1987; 197(3):425-38. DOI: 10.1016/0022-2836(87)90556-0. View

12.

Mennecier F, Daegelen D, Schweighoffer F, Levin M, Kahn A . Expression of aldolase A messenger RNAs in human adult and foetal tissues and in hepatoma. Biochem Biophys Res Commun. 1986; 134(3):1093-100. DOI: 10.1016/0006-291x(86)90363-3. View

13.

Mitchell P, Wang C, Tjian R . Positive and negative regulation of transcription in vitro: enhancer-binding protein AP-2 is inhibited by SV40 T antigen. Cell. 1987; 50(6):847-61. DOI: 10.1016/0092-8674(87)90512-5. View

14.

Johnson P, Landschulz W, Graves B, McKnight S . Identification of a rat liver nuclear protein that binds to the enhancer core element of three animal viruses. Genes Dev. 1987; 1(2):133-46. DOI: 10.1101/gad.1.2.133. View

15.

Bravo R, Neuberg M, Burckhardt J, Almendral J, Wallich R, Muller R . Involvement of common and cell type-specific pathways in c-fos gene control: stable induction of cAMP in macrophages. Cell. 1987; 48(2):251-60. DOI: 10.1016/0092-8674(87)90428-4. View

16.

Kadesch T, Berg P . Effects of the position of the simian virus 40 enhancer on expression of multiple transcription units in a single plasmid. Mol Cell Biol. 1986; 6(7):2593-601. PMC: 367815. DOI: 10.1128/mcb.6.7.2593-2601.1986. View

17.

Angel P, Baumann I, Stein B, Delius H, Rahmsdorf H, Herrlich P . 12-O-tetradecanoyl-phorbol-13-acetate induction of the human collagenase gene is mediated by an inducible enhancer element located in the 5'-flanking region. Mol Cell Biol. 1987; 7(6):2256-66. PMC: 365350. DOI: 10.1128/mcb.7.6.2256-2266.1987. View

18.

Lee W, Mitchell P, Tjian R . Purified transcription factor AP-1 interacts with TPA-inducible enhancer elements. Cell. 1987; 49(6):741-52. DOI: 10.1016/0092-8674(87)90612-x. View

19.

Atchison M, PERRY R . Tandem kappa immunoglobulin promoters are equally active in the presence of the kappa enhancer: implications for models of enhancer function. Cell. 1986; 46(2):253-62. DOI: 10.1016/0092-8674(86)90742-7. View

20.

Schibler U, Sierra F . Alternative promoters in developmental gene expression. Annu Rev Genet. 1987; 21:237-57. DOI: 10.1146/annurev.ge.21.120187.001321. View