Dysregulation of CREB Binding Protein Triggers Thrombin-induced Proliferation of Vascular Smooth Muscle Cells

Overview

Journal Mol Cell Biochem

Publisher Springer

Specialty Biochemistry

Date 2008 May 23

PMID 18496732

Citations 4

Authors

Jing Chen

Hong Jiang

Lin Xu

Li-Hua Zhu

Lang Wang

Hua-Zhi Wen

Xiao-Rong Hu

Affiliations

Soon will be listed here.

Abstract

Thrombin is a potent mitogen for vascular smooth muscle cells (VSMCs). CBP has been regarded as a potential therapeutic target on the basis of its ability to affect cell growth. Therefore we hypothesized that CBP mediates thrombin-induced proliferation of VSMCs. We constructed recombinant adenoviral vector that expresses four short hairpin RNA (shRNA) targeting rat CBP mRNA (CBP-shRNA/Ad). VSMCs were infected with CBP-shRNA/Ad and treated with thrombin. CBP level were analyzed by quantitative real-time PCR and Western blot. To evaluate VSMC proliferation, the cell cycle and DNA synthesis were analyzed by flow cytometry and (3)H-thymidine incorporation, respectively. CBP-shRNA/Ad infection inhibited thrombin-induced CBP expression in a dose-dependent manner concomitant with a decrease in the percentage of cells in the S phase and in DNA synthesis. These findings suggest that CBP plays a pivotal role in the S phase progression of VSMCs.

Citing Articles

Transcriptional regulation of platelet-derived growth factor-B chain by thrombin in endothelial cells: involvement of Egr-1 and CREB-binding protein.

Chen J, Xu L, Chen S, Yang J, Jiang H Mol Cell Biochem. 2012; 366(1-2):81-7.

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CBP knockdown inhibits angiotensin II-induced vascular smooth muscle cells proliferation through downregulating NF-kB transcriptional activity.

Yang J, Jiang H, Chen S, Chen J, Xu S, Li W Mol Cell Biochem. 2010; 340(1-2):55-62.

PMID: 20157765 DOI: 10.1007/s11010-010-0400-2.

Downregulation of CREB-binding protein expression sensitizes endothelial cells to serum-deprived apoptosis: important role of nitric oxide.

Chen J, Jiang H, Zhu L, Wang L, Xu L Mol Cell Biochem. 2009; 337(1-2):159-66.

PMID: 19851832 DOI: 10.1007/s11010-009-0295-y.

Chronic exposure to fibrin and fibrinogen differentially regulates intracellular Ca2+ in human pulmonary arterial smooth muscle and endothelial cells.

Firth A, Yau J, White A, Chiles P, Marsh J, Morris T Am J Physiol Lung Cell Mol Physiol. 2009; 296(6):L979-86.

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