Formation of Guanosine(5')tetraphospho(5')adenosine Cap Structure by an Unconventional MRNA Capping Enzyme of Vesicular Stomatitis Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2008 May 23

PMID 18495767

Citations 23

Authors

Tomoaki Ogino

Amiya K Banerjee

Affiliations

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Abstract

The RNA-dependent RNA polymerase L protein of vesicular stomatitis virus (VSV) elicits GTPase and RNA:GDP polyribonucleotidyltransferase (PRNTase) activities to produce a 5'-cap core structure, guanosine(5')triphospho(5')adenosine (GpppA), on viral mRNAs. Here, we report that the L protein produces an unusual cap structure, guanosine(5')tetraphospho(5')adenosine (GppppA), that is formed by the transfer of the 5'-monophosphorylated viral mRNA start sequence to GTP by the PRNTase activity before the removal of the gamma-phosphate from GTP by GTPase. Interestingly, GppppA-capped and polyadenylated full-length mRNAs were also found to be synthesized by an in vitro transcription system with the native VSV RNP.

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