Rat Disc Torsional Mechanics: Effect of Lumbar and Caudal Levels and Axial Compression Load

Overview

Journal Spine J

Specialty Orthopedics

Date 2008 May 23

PMID 18495544

Citations 18

Authors

Alejandro A Espinoza Orias

Neil R Malhotra

Dawn M Elliott

Affiliations

Soon will be listed here.

Abstract

Background Context: Rat models with altered loading are used to study disc degeneration and mechano-transduction. Given the prominent role of mechanics in disc function and degeneration, it is critical to measure mechanical behavior to evaluate changes after model interventions. Axial compression mechanics of the rat disc are representative of the human disc when normalized by geometry, and differences between the lumbar and caudal disc have been quantified in axial compression. No study has quantified rat disc torsional mechanics.

Purpose: Compare the torsional mechanical behavior of rat lumbar and caudal discs, determine the contribution of combined axial load on torsional mechanics, and compare the torsional properties of rat discs to human lumbar discs.

Study Design: Cadaveric biomechanical study.

Methods: Cyclic torsion without compressive load followed by cyclic torsion with a fixed compressive load was applied to rat lumbar and caudal disc levels.

Results: The apparent torsional modulus was higher in the lumbar region than in the caudal region: 0.081+/-0.026 (MPa/degrees, mean+/-SD) for lumbar axially loaded; 0.066+/-0.028 for caudal axially loaded; 0.091+/-0.033 for lumbar in pure torsion; and 0.056+/-0.035 for caudal in pure torsion. These values were similar to human disc properties reported in the literature ranging from 0.024 to 0.21 MPa/degrees.

Conclusions: Use of the caudal disc as a model may be appropriate if the mechanical focus is within the linear region of the loading regime. These results provide support for use of this animal model in basic science studies with respect to torsional mechanics.

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References

Zimmerman M, Parsons J, CARTER F, Gutteling E, Lee C, Langrana N . The mechanical properties of the canine lumbar disc and motion segment. Spine (Phila Pa 1976). 1992; 17(2):213-20. DOI: 10.1097/00007632-199202000-00016. View

Haughton V, Lim T, An H . Intervertebral disk appearance correlated with stiffness of lumbar spinal motion segments. AJNR Am J Neuroradiol. 1999; 20(6):1161-5. PMC: 7056248. View

Elliott D, Yerramalli C, Beckstein J, Boxberger J, Johannessen W, Vresilovic E . The effect of relative needle diameter in puncture and sham injection animal models of degeneration. Spine (Phila Pa 1976). 2008; 33(6):588-96. DOI: 10.1097/BRS.0b013e318166e0a2. View

Gardner-Morse M, Stokes I . Structural behavior of human lumbar spinal motion segments. J Biomech. 2004; 37(2):205-12. DOI: 10.1016/j.jbiomech.2003.10.003. View

Hunter C, Matyas J, Duncan N . Cytomorphology of notochordal and chondrocytic cells from the nucleus pulposus: a species comparison. J Anat. 2004; 205(5):357-62. PMC: 1571361. DOI: 10.1111/j.0021-8782.2004.00352.x. View

Ching C, Chow D, Yao F, Holmes A . The effect of cyclic compression on the mechanical properties of the inter-vertebral disc: an in vivo study in a rat tail model. Clin Biomech (Bristol). 2003; 18(3):182-9. DOI: 10.1016/s0268-0033(02)00188-2. View

Ochia R, Inoue N, Takatori R, Andersson G, An H . In vivo measurements of lumbar segmental motion during axial rotation in asymptomatic and chronic low back pain male subjects. Spine (Phila Pa 1976). 2007; 32(13):1394-9. DOI: 10.1097/BRS.0b013e318060122b. View

Fujiwara A, Lim T, An H, Tanaka N, Jeon C, Andersson G . The effect of disc degeneration and facet joint osteoarthritis on the segmental flexibility of the lumbar spine. Spine (Phila Pa 1976). 2001; 25(23):3036-44. DOI: 10.1097/00007632-200012010-00011. View

Stokes I, Iatridis J . Mechanical conditions that accelerate intervertebral disc degeneration: overload versus immobilization. Spine (Phila Pa 1976). 2004; 29(23):2724-32. PMC: 7173624. DOI: 10.1097/01.brs.0000146049.52152.da. View

10.

Crevensten G, Walsh A, Ananthakrishnan D, Page P, Wahba G, Lotz J . Intervertebral disc cell therapy for regeneration: mesenchymal stem cell implantation in rat intervertebral discs. Ann Biomed Eng. 2004; 32(3):430-4. DOI: 10.1023/b:abme.0000017545.84833.7c. View

11.

Drake J, Aultman C, McGill S, Callaghan J . The influence of static axial torque in combined loading on intervertebral joint failure mechanics using a porcine model. Clin Biomech (Bristol). 2005; 20(10):1038-45. DOI: 10.1016/j.clinbiomech.2005.06.007. View

12.

Beckstein J, Sen S, Schaer T, Vresilovic E, Elliott D . Comparison of animal discs used in disc research to human lumbar disc: axial compression mechanics and glycosaminoglycan content. Spine (Phila Pa 1976). 2008; 33(6):E166-73. DOI: 10.1097/BRS.0b013e318166e001. View

13.

Boxberger J, Sen S, Yerramalli C, Elliott D . Nucleus pulposus glycosaminoglycan content is correlated with axial mechanics in rat lumbar motion segments. J Orthop Res. 2006; 24(9):1906-15. DOI: 10.1002/jor.20221. View

14.

Liu Y, Goel V, Dejong A, Njus G, Nishiyama K, Buckwalter J . Torsional fatigue of the lumbar intervertebral joints. Spine (Phila Pa 1976). 1985; 10(10):894-900. DOI: 10.1097/00007632-198512000-00006. View

15.

Thompson R, Pearcy M, Barker T . The mechanical effects of intervertebral disc lesions. Clin Biomech (Bristol). 2004; 19(5):448-55. DOI: 10.1016/j.clinbiomech.2004.01.012. View

16.

Abumi K, Panjabi M, Kramer K, Duranceau J, Oxland T, Crisco J . Biomechanical evaluation of lumbar spinal stability after graded facetectomies. Spine (Phila Pa 1976). 1990; 15(11):1142-7. DOI: 10.1097/00007632-199011010-00011. View

17.

Wilke H, Kavanagh S, Neller S, Haid C, Claes L . Effect of a prosthetic disc nucleus on the mobility and disc height of the L4-5 intervertebral disc postnucleotomy. J Neurosurg. 2001; 95(2 Suppl):208-14. DOI: 10.3171/spi.2001.95.2.0208. View

18.

Elliott D, Sarver J . Young investigator award winner: validation of the mouse and rat disc as mechanical models of the human lumbar disc. Spine (Phila Pa 1976). 2004; 29(7):713-22. DOI: 10.1097/01.brs.0000116982.19331.ea. View

19.

McGlashen K, Miller J, Schultz A, Andersson G . Load displacement behavior of the human lumbo-sacral joint. J Orthop Res. 1987; 5(4):488-96. DOI: 10.1002/jor.1100050404. View

20.

OConnell G, Vresilovic E, Elliott D . Comparison of animals used in disc research to human lumbar disc geometry. Spine (Phila Pa 1976). 2007; 32(3):328-33. DOI: 10.1097/01.brs.0000253961.40910.c1. View