Formation of Potential Titanium Antigens Based on Protein Binding to Titanium Dioxide Nanoparticles

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2008 May 21

PMID 18488417

Citations 8

Authors

Carmen Irina Vamanu

Paul Johan Hol

Zouhir Ekeland Allouni

Said Elsayed

Nils Roar Gjerdet

Affiliations

Soon will be listed here.

Abstract

Degradation products of titanium implants include free ions, organo-metallic complexes, and particles, ranging from nano to macro sizes. The biological effects, especially of nanoparticles, is yet unknown. The main objective of this study was to develop Ti-protein antigens in physiological solutions that can be used in testing of cellular responses. For this purpose, 0.1% TiO2 nanoparticles less than 100 nm were mixed with human serum albumin (HSA), 0.1% and 1%, in cell culture medium (DMEM, pH 7.2). The Ti concentrations in the resulting solutions were analyzed by inductively coupled plasma mass spectrometry. The stability of the nanoparticles in suspension was analyzed by UV-vis spectrophotometer and Dynamic Light Scattering. The concentration of Ti in suspension was dependent on the presence and concentration of HSA. Albumin prevented high aggregation rate of TiO2 nanoparticles in cell culture medium. It is shown that nano TiO2-protein stable aggregates can be produced under physiological conditions at high concentrations, and are candidates for use in cellular tests.

Citing Articles

Metal nanomaterials: Immune effects and implications of physicochemical properties on sensitization, elicitation, and exacerbation of allergic disease.

Roach K, Stefaniak A, Roberts J J Immunotoxicol. 2019; 16(1):87-124.

PMID: 31195861 PMC: 6649684. DOI: 10.1080/1547691X.2019.1605553.

Novel Nanoparticulate and Ionic Titanium Antigens for Hypersensitivity Testing.

Hol P, Kristoffersen E, Gjerdet N, Pellowe A Int J Mol Sci. 2018; 19(4).

PMID: 29642398 PMC: 5979587. DOI: 10.3390/ijms19041101.

Cell-based cytotoxicity assays for engineered nanomaterials safety screening: exposure of adipose derived stromal cells to titanium dioxide nanoparticles.

Xu Y, Hadjiargyrou M, Rafailovich M, Mironava T J Nanobiotechnology. 2017; 15(1):50.

PMID: 28693576 PMC: 5504822. DOI: 10.1186/s12951-017-0285-2.

Exposure to TiO2 nanoparticles increases Staphylococcus aureus infection of HeLa cells.

Xu Y, Wei M, Ou-Yang H, Walker S, Wang H, Gordon C J Nanobiotechnology. 2016; 14:34.

PMID: 27102228 PMC: 4840899. DOI: 10.1186/s12951-016-0184-y.

The effect of blood protein adsorption on cellular uptake of anatase TiO2 nanoparticles.

Allouni Z, Gjerdet N, Cimpan M, Hol P Int J Nanomedicine. 2015; 10:687-95.

PMID: 25632230 PMC: 4304597. DOI: 10.2147/IJN.S72726.

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