Cell Density Modulates the Decrease of Cytosolic Free Ca2+ Induced by Atrial Natriuretic Hormone, S-nitroso-N-acetylpenicillamine and 8-bromo Cyclic GMP in Cultured Rat Mesangial Cells

Overview

Journal Biochem J

Specialty Biochemistry

Date 1991 Mar 1

PMID 1848751

Citations 4

Authors

F Lermioglu

J Goyal

A Hassid

Affiliations

Soon will be listed here.

Abstract

Cyclic GMP-elevating agents, including atrial natriuretic hormone and NO-generating vasodilators, decrease cytosolic free Ca2+ levels in mesangial cells. We have investigated the role of cell density as a modulator of the decrease in cytosolic free Ca2+ induced by the cyclic GMP (cGMP)-elevating vasodilators atrial natriuretic peptide (99-126) [ANP (99-126); 'atriopeptin 28'] and the NO-generating vasodilator S-nitroso-N-acetylpenicillamine (SNAP), in cultured rat mesangial cells. Increasing cell density was significantly correlated with the decrease in cytosolic free Ca2+ induced by ANP (99-126) or SNAP. Moreover, this effect was independent of the cells' proliferative status. ANP (99-126) and SNAP induced greater fold stimulation of cGMP accumulation in high-density cells, but the levels of cGMP elicited by high concentrations of ANP (99-126) or SNAP were similar in high- and low-density cells. 8-Bromo cGMP was more effective in decreasing cytosolic free Ca2+ in high- than in low-density cells, suggesting that the greater effectiveness of ANP (99-126) and SNAP was, in part, due to greater effectiveness of endogenous cGMP in high-density cells. The results document that cell density, but not proliferative status, plays an important role in the modulation of intracellular Ca2+ dynamics in rat mesangial cells by atriopeptins, NO-generating vasodilators and cGMP.

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References

Palmer R, Ferrige A, Moncada S . Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature. 1987; 327(6122):524-6. DOI: 10.1038/327524a0. View

Brooker G, Harper J, Terasaki W, Moylan R . Radioimmunoassay of cyclic AMP and cyclic GMP. Adv Cyclic Nucleotide Res. 1979; 10:1-33. View

Ignarro L, Buga G, Wood K, Byrns R, Chaudhuri G . Endothelium-derived relaxing factor produced and released from artery and vein is nitric oxide. Proc Natl Acad Sci U S A. 1987; 84(24):9265-9. PMC: 299734. DOI: 10.1073/pnas.84.24.9265. View

Ballermann B, Hoover R, Karnovsky M, Brenner B . Physiologic regulation of atrial natriuretic peptide receptors in rat renal glomeruli. J Clin Invest. 1985; 76(6):2049-56. PMC: 424304. DOI: 10.1172/JCI112207. View

Appel R . Growth inhibitory activity of atrial natriuretic factor in rat glomerular mesangial cells. FEBS Lett. 1988; 238(1):135-8. DOI: 10.1016/0014-5793(88)80242-4. View

LARAGH J, Atlas S . Atrial natriuretic hormone: a regulator of blood pressure and volume homeostasis. Kidney Int Suppl. 1988; 25:S64-71. View

Appel R, Dubyak G, Dunn M . Effect of atrial natriuretic factor on cytosolic free calcium in rat glomerular mesangial cells. FEBS Lett. 1987; 224(2):396-400. DOI: 10.1016/0014-5793(87)80491-x. View

Hassid A . Atriopeptins decrease resting and hormone-elevated cytosolic Ca in cultured mesangial cells. Am J Physiol. 1987; 253(6 Pt 2):F1077-82. DOI: 10.1152/ajprenal.1987.253.6.F1077. View

Appel R, Wang J, Simonson M, Dunn M . A mechanism by which atrial natriuretic factor mediates its glomerular actions. Am J Physiol. 1986; 251(6 Pt 2):F1036-42. DOI: 10.1152/ajprenal.1986.251.6.F1036. View

10.

Bianchi C, Gutkowska J, Thibault G, Garcia R, Genest J, Cantin M . Distinct localization of atrial natriuretic factor and angiotensin II binding sites in the glomerulus. Am J Physiol. 1986; 251(4 Pt 2):F594-602. DOI: 10.1152/ajprenal.1986.251.4.F594. View

11.

Kawase T, Ishikawa I, Suzuki A . NaF-induced Ca2+ mobilization is dependent upon the culture density in a parathyroid hormone-responsive osteoblast-like cell line. Life Sci. 1988; 43(26):2241-7. DOI: 10.1016/0024-3205(88)90417-1. View

12.

Sedor J, Abboud H . Histamine modulates contraction and cyclic nucleotides in cultured rat mesangial cells. Differential effects mediated by histamine H1 and H2 receptors. J Clin Invest. 1985; 75(5):1679-89. PMC: 425511. DOI: 10.1172/JCI111876. View

13.

Kimura H, Murad F . Localization of particulate guanylate cyclase in plasma membranes and microsomes of rat liver. J Biol Chem. 1975; 250(12):4810-7. View

14.

Garg U, Hassid A . Inhibition of rat mesangial cell mitogenesis by nitric oxide-generating vasodilators. Am J Physiol. 1989; 257(1 Pt 2):F60-6. DOI: 10.1152/ajprenal.1989.257.1.F60. View

15.

Singhal P, DeCandido S, Satriano J, Schlondorff D, Hays R . Atrial natriuretic peptide and nitroprusside cause relaxation of cultured rat mesangial cells. Am J Physiol. 1989; 257(1 Pt 1):C86-93. DOI: 10.1152/ajpcell.1989.257.1.C86. View

16.

Goodman L, Majack R . Vascular smooth muscle cells express distinct transforming growth factor-beta receptor phenotypes as a function of cell density in culture. J Biol Chem. 1989; 264(9):5241-4. View

17.

Inagami T . Atrial natriuretic factor. J Biol Chem. 1989; 264(6):3043-6. View

18.

Abell T, Richards A, Ikram H, Espiner E, Yandle T . Atrial natriuretic factor inhibits proliferation of vascular smooth muscle cells stimulated by platelet-derived growth factor. Biochem Biophys Res Commun. 1989; 160(3):1392-6. DOI: 10.1016/s0006-291x(89)80158-5. View

19.

NEEDLEMAN P, Blaine E, Greenwald J, Michener M, Saper C, Stockmann P . The biochemical pharmacology of atrial peptides. Annu Rev Pharmacol Toxicol. 1989; 29:23-54. DOI: 10.1146/annurev.pa.29.040189.000323. View

20.

Campbell J, Kocher O, Skalli O, Gabbiani G, Campbell G . Cytodifferentiation and expression of alpha-smooth muscle actin mRNA and protein during primary culture of aortic smooth muscle cells. Correlation with cell density and proliferative state. Arteriosclerosis. 1989; 9(5):633-43. DOI: 10.1161/01.atv.9.5.633. View