Transformation-defective V-ski Induces MyoD and Myogenin Expression but Not Myotube Formation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1991 Feb 1

PMID 1846665

Citations 17

Authors

C Colmenares

J K Teumer

E Stavnezer

Affiliations

Soon will be listed here.

Abstract

The ski oncogene induces muscle differentiation in otherwise nonmyogenic quail embryo cells (C. Colmenares and E. Stavnezer, Cell 59:293-303, 1989). Here we report that v-ski induces both MyoD and myogenin expression, suggesting that activation of these muscle regulatory genes may be a critical step in ski-induced myogenesis. We also describe a transformation-defective mutant of v-ski (tdM5i) that fails to induce myotube formation, although it induces the expression of many muscle-specific genes, including the MyoD and myogenin genes. Therefore, if activation of MyoD and myogenin expression is a necessary component of the myogenic program triggered by ski, it is clearly insufficient to account for complete muscle differentiation.

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References

Edmondson D, Olson E . A gene with homology to the myc similarity region of MyoD1 is expressed during myogenesis and is sufficient to activate the muscle differentiation program. Genes Dev. 1989; 3(5):628-40. DOI: 10.1101/gad.3.5.628. View

EMERSON Jr C, Beckner S . Activation of myosin synthesis in fusing and mononucleated myoblasts. J Mol Biol. 1975; 93(4):431-47. DOI: 10.1016/0022-2836(75)90238-7. View

Rhodes S, Konieczny S . Identification of MRF4: a new member of the muscle regulatory factor gene family. Genes Dev. 1989; 3(12B):2050-61. DOI: 10.1101/gad.3.12b.2050. View

Berg J . Potential metal-binding domains in nucleic acid binding proteins. Science. 1986; 232(4749):485-7. DOI: 10.1126/science.2421409. View

Barkas A, Brodeur D, Stavnezer E . Polyproteins containing a domain encoded by the V-SKI oncogene are located in the nuclei of SKV-transformed cells. Virology. 1986; 151(1):131-8. DOI: 10.1016/0042-6822(86)90111-x. View

Freedman L, Luisi B, Korszun Z, Basavappa R, SIGLER P, Yamamoto K . The function and structure of the metal coordination sites within the glucocorticoid receptor DNA binding domain. Nature. 1988; 334(6182):543-6. DOI: 10.1038/334543a0. View

Akhurst R, Flavin N, Worden J . Isolation and characterization of a variant myoblast cell line that is temperature sensitive for differentiation. Mol Cell Biol. 1988; 8(6):2335-41. PMC: 363431. DOI: 10.1128/mcb.8.6.2335-2341.1988. View

Lassar A, Buskin J, Lockshon D, Davis R, Apone S, Hauschka S . MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer. Cell. 1989; 58(5):823-31. DOI: 10.1016/0092-8674(89)90935-5. View

Wright W, Sassoon D, Lin V . Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. Cell. 1989; 56(4):607-17. DOI: 10.1016/0092-8674(89)90583-7. View

10.

Murre C, McCaw P, Vaessin H, Caudy M, Jan L, Jan Y . Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell. 1989; 58(3):537-44. DOI: 10.1016/0092-8674(89)90434-0. View

11.

Murre C, McCaw P, Baltimore D . A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell. 1989; 56(5):777-83. DOI: 10.1016/0092-8674(89)90682-x. View

12.

Stavnezer E, Brodeur D, Brennan L . The v-ski oncogene encodes a truncated set of c-ski coding exons with limited sequence and structural relatedness to v-myc. Mol Cell Biol. 1989; 9(9):4038-45. PMC: 362468. DOI: 10.1128/mcb.9.9.4038-4045.1989. View

13.

Weintraub H, Tapscott S, Davis R, Thayer M, Adam M, Lassar A . Activation of muscle-specific genes in pigment, nerve, fat, liver, and fibroblast cell lines by forced expression of MyoD. Proc Natl Acad Sci U S A. 1989; 86(14):5434-8. PMC: 297637. DOI: 10.1073/pnas.86.14.5434. View

14.

Braun T, Bober E, Tannich E, Arnold H . A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts. EMBO J. 1989; 8(3):701-9. PMC: 400865. DOI: 10.1002/j.1460-2075.1989.tb03429.x. View

15.

Kaufman S, FOSTER R . Replicating myoblasts express a muscle-specific phenotype. Proc Natl Acad Sci U S A. 1988; 85(24):9606-10. PMC: 282812. DOI: 10.1073/pnas.85.24.9606. View

16.

Davis R, Weintraub H, Lassar A . Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell. 1987; 51(6):987-1000. DOI: 10.1016/0092-8674(87)90585-x. View

17.

Peterson C, Gordon H, Hall Z, Paterson B, Blau H . Negative control of the helix-loop-helix family of myogenic regulators in the NFB mutant. Cell. 1990; 62(3):493-502. DOI: 10.1016/0092-8674(90)90014-6. View

18.

Schafer B, Blakely B, Darlington G, Blau H . Effect of cell history on response to helix-loop-helix family of myogenic regulators. Nature. 1990; 344(6265):454-8. DOI: 10.1038/344454a0. View

19.

Benezra R, Davis R, Lockshon D, Turner D, Weintraub H . The protein Id: a negative regulator of helix-loop-helix DNA binding proteins. Cell. 1990; 61(1):49-59. DOI: 10.1016/0092-8674(90)90214-y. View

20.

Davis R, Cheng P, Lassar A, Weintraub H . The MyoD DNA binding domain contains a recognition code for muscle-specific gene activation. Cell. 1990; 60(5):733-46. DOI: 10.1016/0092-8674(90)90088-v. View