Novel Downstream Elements Upregulate Transcription Initiated from an Epstein-Barr Virus Latent Promoter

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1991 Jan 1

PMID 1846596

Citations 11

Authors

D Walls

M Perricaudet

Affiliations

Soon will be listed here.

Abstract

The modulation of nuclear antigen expression from the Epstein-Barr virus (EBV) genome in virus-infected cells is crucial to the establishment and maintenance of the immortalized state. The EBV nuclear antigen (EBNA) genes are all transcribed from either of two promoters whose alternate usage is not understood. We have studied the EBNA transcriptional domain by using various promoter constructs to transfect a variety of EBV positive cell lines and then measured promoter activity by RNase protection analysis and nuclear run-on transcription assay. These experiments have shown that at least two distinct and novel DNA sequence elements are located in the intronic region situated between both promoters and that their presence results in a 100-fold stimulation of transcription initiated from the upstream promoter. One of these elements is shown to bind nuclear proteins from an EBV-positive cell line. In addition, an important sequence homology is noted between this element and the core sequences of a number of well known viral enhancers. Taken together, the results show that EBNA transcription is controlled by upstream and intronic elements in a regulatory domain that is at least 7 kb long.

Citing Articles

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Methylation status of the Epstein-Barr virus (EBV) BamHI W latent cycle promoter and promoter activity: analysis with novel EBV-positive Burkitt and lymphoblastoid cell lines.

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The amino acid region 248-382 of the Epstein-Barr virus nuclear protein 2 (EBNA2) is responsible for the EBNA2-induced EBV reactivation.

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Protein-DNA binding and CpG methylation at nucleotide resolution of latency-associated promoters Qp, Cp, and LMP1p of Epstein-Barr virus.

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PMID: 10233991 PMC: 112573. DOI: 10.1128/JVI.73.6.5214-5219.1999.

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