Microeukaryote Diversity in a Marine Methanol-fed Fluidized Denitrification System

Overview

Journal Microb Ecol

Specialties Environmental Health
Microbiology

Date 2008 May 10

PMID 18465167

Citations 5

Authors

Veronique Laurin

Normand Labbe

Serge Parent

Pierre Juteau

Richard Villemur

Affiliations

Soon will be listed here.

Abstract

The marine methanol-fed fluidized denitrification system operated by the Montreal Biodome includes carriers on which a denitrifying biofilm has developed. Previous observations showed a high abundance of microeukaryotes living in and around the biofilm. These eukaryotes may influence the system's denitrification efficiency. The composition of the microeukaryote population was determined. Microscopic observations showed at least 20 different morphologies that included large numbers of ciliates. Molecular analyses of an 18S ribosomal RNA (rDNA) gene library revealed 31 different phylotypes. Alveolobiontes were the most abundant phylotypes and made up 75% of the 159 screened clones. Other eukaryotic groups, including Stramenopiles, Fungi, Amoebozoa, and nematodes, were also present. From 18S rDNA specific sequences, one of the Amoebozoa-affiliated phylotypes was visualized by fluorescence in situ hybridization. It had a rod-like irregular shape and measured less than 5 mum in length. We determined the impact of protozoans on the denitrifying activity. In a laboratory-scale batch culture assays, the denitrifying biofilm was treated with cycloheximide and nystatin that eliminated the protozoans. No difference in the denitrification rate was found. However, planktonic bacteria were more abundant in the treated culture medium.

Citing Articles

The bacterial strains JAM1 and GP59 of the species differ in their expression profiles of denitrification genes in oxic and anoxic cultures.

Lestin L, Villemur R PeerJ. 2024; 12:e18361.

PMID: 39484211 PMC: 11526790. DOI: 10.7717/peerj.18361.

Coronamoeba villafranca gen. nov. sp. nov. (Amoebozoa, Dermamoebida) challenges the correlation of morphology and phylogeny in Amoebozoa.

Kudryavtsev A, Voytinsky F, Volkova E Sci Rep. 2022; 12(1):12541.

PMID: 35869259 PMC: 9307759. DOI: 10.1038/s41598-022-16721-2.

Strain-level genetic diversity of confers plasticity to denitrification capacity in a methylotrophic marine denitrifying biofilm.

Geoffroy V, Payette G, Mauffrey F, Lestin L, Constant P, Villemur R PeerJ. 2018; 6:e4679.

PMID: 29707436 PMC: 5918138. DOI: 10.7717/peerj.4679.

Denitrifying metabolism of the methylotrophic marine bacterium strain JAM1.

Mauffrey F, Cucaita A, Constant P, Villemur R PeerJ. 2017; 5:e4098.

PMID: 29201569 PMC: 5710167. DOI: 10.7717/peerj.4098.

A mesocosm study of the changes in marine flagellate and ciliate communities in a crude oil bioremediation trial.

Gertler C, Nather D, Gerdts G, Malpass M, Golyshin P Microb Ecol. 2010; 60(1):180-91.

PMID: 20393846 DOI: 10.1007/s00248-010-9660-3.

References

Richards T, Vepritskiy A, Gouliamova D, Nierzwicki-Bauer S . The molecular diversity of freshwater picoeukaryotes from an oligotrophic lake reveals diverse, distinctive and globally dispersed lineages. Environ Microbiol. 2005; 7(9):1413-25. DOI: 10.1111/j.1462-2920.2005.00828.x. View

Huber T, Faulkner G, Hugenholtz P . Bellerophon: a program to detect chimeric sequences in multiple sequence alignments. Bioinformatics. 2004; 20(14):2317-9. DOI: 10.1093/bioinformatics/bth226. View

Risgaard-Petersen N, Langezaal A, Ingvardsen S, Schmid M, Jetten M, Op den Camp H . Evidence for complete denitrification in a benthic foraminifer. Nature. 2006; 443(7107):93-6. DOI: 10.1038/nature05070. View

Wong F, Carson J, Elliott N . 18S ribosomal DNA-based PCR identification of Neoparamoeba pemaquidensis, the agent of amoebic gill disease in sea-farmed salmonids. Dis Aquat Organ. 2004; 60(1):65-76. DOI: 10.3354/dao060065. View

Labbe N, Parent S, Villemur R . Addition of trace metals increases denitrification rate in closed marine systems. Water Res. 2003; 37(4):914-20. DOI: 10.1016/s0043-1354(02)00383-4. View

Moon-van der Staay S, De Wachter R, Vaulot D . Oceanic 18S rDNA sequences from picoplankton reveal unsuspected eukaryotic diversity. Nature. 2001; 409(6820):607-10. DOI: 10.1038/35054541. View

Butinar L, Santos S, Spencer-Martins I, Oren A, Gunde-Cimerman N . Yeast diversity in hypersaline habitats. FEMS Microbiol Lett. 2005; 244(2):229-34. DOI: 10.1016/j.femsle.2005.01.043. View

Dawson S, Pace N . Novel kingdom-level eukaryotic diversity in anoxic environments. Proc Natl Acad Sci U S A. 2002; 99(12):8324-9. PMC: 123066. DOI: 10.1073/pnas.062169599. View

Stoeck T, Hayward B, Taylor G, Varela R, Epstein S . A multiple PCR-primer approach to access the microeukaryotic diversity in environmental samples. Protist. 2006; 157(1):31-43. DOI: 10.1016/j.protis.2005.10.004. View

10.

Dolan J . Marine ecology: different measures of biodiversity. Nature. 2005; 433(7024):E9. DOI: 10.1038/nature03320. View

11.

Stoeck T, Epstein S . Novel eukaryotic lineages inferred from small-subunit rRNA analyses of oxygen-depleted marine environments. Appl Environ Microbiol. 2003; 69(5):2657-63. PMC: 154554. DOI: 10.1128/AEM.69.5.2657-2663.2003. View

12.

van Hannen E, MOOIJ W, van Agterveld M, Gons H, Laanbroek H . Detritus-dependent development of the microbial community in an experimental system: qualitative analysis by denaturing gradient gel electrophoresis. Appl Environ Microbiol. 1999; 65(6):2478-84. PMC: 91365. DOI: 10.1128/AEM.65.6.2478-2484.1999. View

13.

Lopez-Garcia P, Philippe H, Gail F, Moreira D . Autochthonous eukaryotic diversity in hydrothermal sediment and experimental microcolonizers at the Mid-Atlantic Ridge. Proc Natl Acad Sci U S A. 2003; 100(2):697-702. PMC: 141059. DOI: 10.1073/pnas.0235779100. View

14.

Martin-Cereceda M, Zamora J, Perez-Uz B, Guinea A . Ciliate communities of rotating biological contactor biofilms: a multivariate approach. Syst Appl Microbiol. 2002; 25(2):301-13. DOI: 10.1078/0723-2020-00090. View

15.

Jee B, Kim Y, Park M . Morphology and biology of parasite responsible for scuticociliatosis of cultured olive flounder Paralichthys olivaceus. Dis Aquat Organ. 2002; 47(1):49-55. DOI: 10.3354/dao047049. View

16.

Luna-Pabello V, Paul E, Duran De Bazua C . Ciliatological characterization of a biological reactor that eliminates nitrogen with intermitent aeration. Rev Latinoam Microbiol. 1996; 38(2):89-96. View

17.

Aleya L, Hartmann H, Devaux J . Evidence for the contribution of ciliates to denitrification in a eutrophic lake. Eur J Protistol. 2012; 28(3):316-21. DOI: 10.1016/S0932-4739(11)80238-5. View

18.

Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar . ARB: a software environment for sequence data. Nucleic Acids Res. 2004; 32(4):1363-71. PMC: 390282. DOI: 10.1093/nar/gkh293. View

19.

Sudo R, Aiba S . Role and function of protozoa in the biological treatment of polluted waters. Adv Biochem Eng Biotechnol. 1984; 29:117-41. DOI: 10.1007/BFb0000692. View

20.

Labbe N, Juteau P, Parent S, Villemur R . Bacterial diversity in a marine methanol-fed denitrification reactor at the montreal biodome, Canada. Microb Ecol. 2003; 46(1):12-21. DOI: 10.1007/s00248-002-1056-6. View