Redox Activity OfHydrodictyon Reticulatum Plasmalemma Vesicles
Overview
Overview
Journal
Folia Microbiol (Praha)
Publisher
Springer
Specialty
Microbiology
Date
2008 May 8
PMID
18461491
Authors
Authors
Affiliations
Affiliations
Soon will be listed here.
Abstract
Plasmalemma vesicles with preserved redox activity were prepared from nets ofHydrodictyon reticulatum. Since the walls are mechanically very resistant, a combination of partial cell-wall enzyme digestion and ultrasonic homogenization had to be used for the disruption of cells. To isolate the plasma-membrane-enriched microsomal fraction separation in an aqueous two-phase polymer system was found to be most suitable. The right-side-out vesicles reduced added hexacyanoferrate(III) by electrons supplied either by a transmembrane flow or from external NADH.
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