Down-regulation of MyoD Gene Expression in Rat Diaphragm Muscle with Heart Failure

Overview

Journal Int J Exp Pathol

Publisher Wiley

Specialty Pathology

Date 2008 May 8

PMID 18460074

Citations 11

Authors

Francis da Silva Lopes

Robson Francisco Carvalho

Gerson Eduardo Rocha Campos

Mario Matheus Sugizaki

Carlos Roberto Padovani

Celia Regina Nogueira

Antonio Carlos Cicogna

Maeli Dal Pai-Silva

Affiliations

Soon will be listed here.

Abstract

Diaphragm myopathy has been described in patients with heart failure (HF), with alterations in myosin heavy chains (MHC) expression. The pathways that regulate MHC expression during HF have not been described, and myogenic regulatory factors (MRFs) may be involved. The purpose of this investigation was to determine MRF mRNA expression levels in the diaphragm. Diaphragm muscle from both HF and control Wistar rats was studied when overt HF had developed, 22 days after monocrotaline administration. MyoD, myogenin and MRF4 gene expression were determined by RT-PCR and MHC isoforms by polyacrylamide gel electrophoresis. Heart failure animals presented decreased MHC IIa/IIx protein isoform and MyoD gene expression, without altering MHC I, IIb, myogenin and MRF4. Our results show that in HF, MyoD is selectively down-regulated, which might be associated with alterations in MHC IIa/IIx content. These changes are likely to contribute to the diaphragm myopathy caused by HF.

Citing Articles

Preventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertension.

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The conventional isoproterenol-induced heart failure model does not consistently mimic the diaphragmatic dysfunction observed in patients.

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Long non-coding RNA MALAT1 promotes cardiac remodeling in hypertensive rats by inhibiting the transcription of MyoD.

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Preventive aerobic training exerts a cardioprotective effect on rats treated with monocrotaline.

Pacagnelli F, de Almeida Sabela A, Okoshi K, Mariano T, Campos D, Carvalho R Int J Exp Pathol. 2016; 97(3):238-47.

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Fractal Dimension in Quantifying Experimental-Pulmonary-Hypertension-Induced Cardiac Dysfunction in Rats.

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