Transmission Electron Microscope Characterisation of Molar-incisor-hypomineralisation

Overview

Journal J Mater Sci Mater Med

Publisher Springer

Specialty Biomedical Engineering

Date 2008 Apr 29

PMID 18438701

Citations 9

Authors

Zonghan Xie

Nicky M Kilpatrick

Michael V Swain

Paul R Munroe

Mark Hoffman

Affiliations

Soon will be listed here.

Abstract

Molar-incisor-hypomineralisation (MIH), one of the major developmental defects in dental enamel, is presenting challenge to clinicians due, in part, to the limited understanding of microstructural changes in affected teeth. Difficulties in the preparation of site-specific transmission electron microscope (TEM) specimens are partly responsible for this deficit. In this study, a dual-beam field emission scanning electron microscope (FESEM)/focused ion beam (FIB) milling instrument was used to prepare electron transparent specimens of sound and hypomineralised enamel. Microstructural analysis revealed that the hypomineralised areas in enamel were associated with marked changes in microstructure; loosely packed apatite crystals within prisms and wider sheath regions were identified. Microstructural changes appear to occur during enamel maturation and may be responsible for the dramatic reduction in mechanical properties of the affected regions. An enhanced knowledge of the degradation of structural integrity in hypomineralised enamel could shed light on more appropriate management strategies for these developmental defects.

Citing Articles

Analysis of the stomatognathic system functions and mastication muscles in children affected by molar hypomineralization (MH): a cross-sectional study.

Carvalho M, Molena K, Regalo S, Goncalves L, de Paula-Silva F, Vicioni-Marques F Eur Arch Paediatr Dent. 2024; 25(5):669-676.

PMID: 38969936 DOI: 10.1007/s40368-024-00925-3.

Proteomic Analyses Discern the Developmental Inclusion of Albumin in Pig Enamel: A New Model for Human Enamel Hypomineralization.

Gil-Bona A, Karaaslan H, Depalle B, Sulyanto R, Bidlack F Int J Mol Sci. 2023; 24(21).

PMID: 37958567 PMC: 10650821. DOI: 10.3390/ijms242115577.

Electromyographic analysis of the stomatognathic system of children with Molar-incisor hypomineralization.

Carvalho M, Regalo S, Siessere S, Goncalves L, de Paula-Silva F, Vicioni-Marques F PLoS One. 2023; 18(2):e0277030.

PMID: 36827292 PMC: 9955966. DOI: 10.1371/journal.pone.0277030.

Molar-Incisor Hypomineralization: Prevalence Comparative Study in 6 Years of Interval.

Damares Lago J, Restrepo M, Bussaneli D, Patricia Cavalheiro J, de Souza J, Santos-Pinto L ScientificWorldJournal. 2022; 2022:4743252.

PMID: 36530554 PMC: 9757933. DOI: 10.1155/2022/4743252.

Clinical evaluation of giomer- and resin-based fissure sealants on permanent molars affected by molar-incisor hypomineralization: a randomized clinical trial.

Ozgur B, Kargin S, Olmez M BMC Oral Health. 2022; 22(1):275.

PMID: 35790955 PMC: 9258125. DOI: 10.1186/s12903-022-02298-9.

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