In Vitro Evaluation of Experimental Agents for Anti-HIV Activity

Overview

Journal Curr Protoc Immunol

Specialty Allergy & Immunology

Date 2008 Apr 25

PMID 18432713

Citations 6

Authors

D D Richman

V A Johnson

D M Mayers

T Shirasaka

M C OBRIEN

H Mitsuya

Affiliations

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Abstract

This unit presents an assay that has proven useful as an initial screening test is an HIV cytopathic effect (CPE) inhibition assay in which immortalized T cell lines (e.g., ATH8 or MT2) that are profoundly sensitive to the cytopathic effect of certain strains of HIV are utilized as target cells. Additional protocols assess the anti-HIV activity of certain candidate agents by measuring inhibition of syncytium formation or p24 gag protein production by ELISA. Calculation of the 50% inhibitory concentration (IC(50)) is also presented.

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