Protein Identification Using a Quadrupole Ion Trap Mass Spectrometer and SEQUEST Database Matching

Overview

Journal Curr Protoc Protein Sci

Specialties Biochemistry
Pathology

Date 2008 Apr 23

PMID 18429128

Citations 4

Authors

R E Moore

M K Young

T D Lee

Affiliations

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Abstract

A procedure is described for rapid, sensitive protein identification utilizing liquid chromatography--tandem mass spectrometry. The analysis is performed on mixtures of peptides obtained by enzyme digestion. The SEQUEST computer program is used to match the sequence information in the spectra to a database of known protein sequences.

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