» Articles » PMID: 18428528

Assessment of Cell Viability in Primary Neuronal Cultures

Overview
Date 2008 Apr 23
PMID 18428528
Citations 12
Authors
Affiliations
Soon will be listed here.
Abstract

Four commonly used methods for the assessment of neuronal (or glial) cell viability are described in this unit. The MTT assay is sensitive to the function of labile mitochondrial enzymes, which typically lose activity early in the progression towards death. The lactate dehydrogenase (LDH) assay measures the appearance of this cytosolic enzyme in the bathing medium, providing a measure of plasma membrane integrity. Loss of plasma membrane integrity is also the basis of the trypan blue dye assay and the propidium iodide assay. Trypan blue staining is assessed by cell counts; propidium iodide labeling can be assessed either by cell counts, typically in conjunction with fluorescein diacetate counterstaining to identify intact cells containing adequate levels of functional esterases, or with a fluorescence plate reader.

Citing Articles

The WMI Rat of Premature Cognitive Aging Presents Intrinsic Vulnerability to Oxidative Stress in Primary Neurons and Astrocytes Compared to Its Nearly Isogenic WLI Control.

Ferreira A, Harter A, Afreen S, Kanai K, Batori S, Redei E Int J Mol Sci. 2024; 25(3).

PMID: 38338968 PMC: 10855588. DOI: 10.3390/ijms25031692.


Chronic VEGFR-3 signaling preserves dendritic arborization and sensitization under stress.

Chakraborty A, Upadhya R, Usman T, Shetty A, Rutkowski J Brain Behav Immun. 2021; 98:219-233.

PMID: 34389489 PMC: 8511130. DOI: 10.1016/j.bbi.2021.08.007.


Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration.

Joshi D, Zhang C, Babujee L, Vevea J, August B, Sheng Z Cell Rep. 2019; 29(3):685-696.e5.

PMID: 31618636 PMC: 6884150. DOI: 10.1016/j.celrep.2019.09.012.


Once the Light Touch to the Brain: Cytotoxic Effects of Low-Dose Gamma-Ray, Laser Light, and Visible Light on Rat Neuronal Cell Culture.

Cakir M, Colak A, Calikoglu C, Taspinar N, Sagsoz M, Kadioglu H Eurasian J Med. 2016; 48(2):76-83.

PMID: 27551168 PMC: 4970559. DOI: 10.5152/eurasianjmed.2015.0304.


Edaravone prevents neurotoxicity of mutant L166P DJ-1 in Parkinson's disease.

Li B, Yu D, Xu Z J Mol Neurosci. 2013; 51(2):539-49.

PMID: 23657982 DOI: 10.1007/s12031-013-0022-8.