Effects of Simulated Carbon Dioxide and Helium Peumoperitoneum on Proliferation and Apoptosis of Gastric Cancer Cells

Overview

Journal World J Gastroenterol

Publisher Baishideng Publishing Group

Specialty Gastroenterology

Date 2008 Apr 15

PMID 18407602

Citations 3

Authors

Ying-Xue Hao

Hua Zhong

Chao Zhang

Dong-Zu Zeng

Yan Shi

Bo Tang

Pei-Wu Yu

Affiliations

Soon will be listed here.

Abstract

Aim: To investigate the effects of carbon dioxide (CO(2)) and helium insufflation administered at different pressures on the growth and apoptosis of cultured human gastric cancer cells.

Methods: The gastric cancer cells MKN-45 were exposed to a CO(2) and helium environment maintained at different pressures (0, 5, 10 and 15 mmHg). The cells were exposed to simulated pneumoperitoneum environment for 4 h, and pH of the culture media was measured after it was moved to normal conditions for 0, 2, 4, 6 and 8 h. Proliferation viability of MKN-45 was examined by 3-[4,5Dimethylthiazol-2-yl],5-diphenyltetrazolium bromide or triazolyl blue (MTT) assay after it was moved to normal conditions. Apoptotic ratio was measured by Annexin V-FITC/PI double labelled staining.

Results: The pH of media was acid and recovered to normal after 4 h in the CO(2) group while it was basic in the helium group. There was no difference between CO(2) groups (under 10 mmHg ) and control group (P > 0.05) in the proliferative viability of the cells. The cultured cells exposed to 15 mmHg CO(2) environment grew more slowly than control group from 4 to 7 d (P < 0.01 ) while there was no difference from 1 to 3 d (P > 0.05). The proliferative viability in helium group was not obviously different from the control group (P > 0.05). The apoptotic ratio of the cultured cells was markedly higher than that of the control group (P < 0.01) at 10 and 15 mmHg CO(2) insufflation pressure. In helium group, the apoptotic ratio was not obviously different from the control group (P > 0.05).

Conclusion: There is no obvious effect in the proliferation and apoptosis of MKN-45 cells under 10 mmHg CO(2) insufflation pressure and helium in any pressure. Fifteen mmHg CO(2) insufflation pressure can inhibit the proliferation of the cells and improve apoptosis.

Citing Articles

Two clinically relevant pressures of carbon dioxide pneumoperitoneum cause hepatic injury in a rabbit model.

Li J, Liu Y, Ye Z, Liu H, Ou S, Tian F World J Gastroenterol. 2011; 17(31):3652-8.

PMID: 21987614 PMC: 3180024. DOI: 10.3748/wjg.v17.i31.3652.

Hepatic injury induced by carbon dioxide pneumoperitoneum in experimental rats.

Xu G, Liu H, Li J, Wu X, Dai X, Liu Y World J Gastroenterol. 2009; 15(24):3060-4.

PMID: 19554662 PMC: 2702117. DOI: 10.3748/wjg.15.3060.

Effects of HIF-1alpha on human gastric cancer cell apoptosis at different CO(2) pressures.

Hao Y, Zhong H, Yu P, Zhang C, Zeng D, Shi Y Clin Exp Med. 2008; 9(2):139-47.

PMID: 19048182 DOI: 10.1007/s10238-008-0023-z.

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