Different Roles of Galectin-9 Isoforms in Modulating E-selectin Expression and Adhesion Function in LoVo Colon Carcinoma Cells

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2008 Apr 11

PMID 18401566

Citations 14

Authors

Feng Zhang

Minhua Zheng

Ying Qu

Jianfang Li

Jun Ji

Bo Feng

Aiguo Lu

Jianwen Li

Mingliang Wang

Bingya Liu

Affiliations

Soon will be listed here.

Abstract

Galectin-9, a member of galectin family, plays multiple roles in a variety of cellular functions, including cell adhesion, aggregation, and apoptosis. Galectin-9 also has three isoforms (named galectin-9L, galectin-9M, and galectin-9S), but whether these isoforms differ in their functions remains poorly understood. In this study, we showed that transient expression of galectin-9L decreased E-selectin levels, while transient expression of galectin-9M or galectin-9S increased E-selectin levels in LoVo cells, which do not express endogenous galectin-9. We also found that over-expression of three galectin-9 isoforms led to increased attachment of LoVo cells to extracellular matrix proteins respectively, while over-expression of galectin-9M or galectin-9S increased the adhesion of LoVo cells to human umbilical vein endothelial cells in vitro. In summary, these findings indicate that different isoforms of galectin-9 exhibit distinct biological functions.

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