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Internal PH Indicators for Biomolecular NMR

Overview
Journal J Biomol NMR
Publisher Springer
Specialties Molecular Biology
Nuclear Medicine
Date 2008 Apr 10
PMID 18398685
Citations 31
Authors
Olga K Baryshnikova
Thomas C Williams
Brian D Sykes
Affiliations
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Abstract

We describe a non-invasive technique for determining pH in biomolecular NMR sample using buffer components (formate, tris, piperazine, and imidazole) as internal pH indicators, whose (1)H NMR chemical shifts are sensitive to pH in a range from 2.5 to 9.8. This method is suitable for a wide range of applications where samples are handled intensively during NMR titrations or in high throughput analysis in structural genomics or metabolomics.

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